Efficient 3D-CTF correction for cryo-electron tomography using NovaCTF improves subtomogram averaging resolution to 3.4Å.
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TLDR
A user-friendly, computationally-efficient 3D-CTF correction tool, NovaCTF, that is compatible with standard tomogram reconstruction workflows in IMOD is presented and it is found that high-resolution structures can be obtained with much smaller subtomogram averaging datasets than are required using 2D- CTF.About:
This article is published in Journal of Structural Biology.The article was published on 2017-09-01 and is currently open access. It has received 198 citations till now.read more
Citations
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Journal ArticleDOI
Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography.
Felix R. Wagner,Reika Watanabe,Ruud Schampers,Digvijay Singh,Hans Persoon,Miroslava Schaffer,Peter Fruhstorfer,Jürgen M. Plitzko,Elizabeth Villa +8 more
TL;DR: This protocol describes how to prepare thin specimens using FIB milling from frozen cells on grids, which enables direct structural analysis of biomolecules in their native environments, i.e., cells.
Journal ArticleDOI
Subtomogram averaging of COPII assemblies reveals how coat organization dictates membrane shape.
TL;DR: The 4.9 Å resolution cryo-tomography and subtomogram averaging structure of the membrane bound COPII inner coat that was obtained by in vitro reconstitution is presented and regulators like the procollagen receptor TANGO1 modulate this mechanism to determine vesicle shape and size.
Journal ArticleDOI
Toward high-resolution in situ structural biology with cryo-electron tomography and subtomogram averaging
TL;DR: Current frontiers and future challenges in routinely determining high-resolution structures in in situ environments using cryo-ET and STA are reviewed, ranging in optimal cases beyond the nanometer barrier.
Journal ArticleDOI
Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions.
Dongjie Zhu,Xiangxi Wang,Qianglin Fang,James L. Van Etten,Michael G. Rossmann,Zihe Rao,Zihe Rao,Xinzheng Zhang +7 more
TL;DR: A block-based reconstruction method to deal with the depth of field effect is presented and it is shown that this approach can improve the resolution of cryo-EM virus structures.
Journal ArticleDOI
AI-based structure prediction empowers integrative structural analysis of human nuclear pores
Shyamal Mosalaganti,Agnieszka Obarska-Kosinska,Marc Siggel,Reiya Taniguchi,Beata Turonova,Christian E. Zimmerli,Katarzyna Buczak,Florian H. Schmidt,Erica Margiotta,Marie-Therese Mackmull,Wim J. H. Hagen,Gerhard Hummer,Jan Kosinski,Martin Beck +13 more
TL;DR: This study used artificial intelligence (AI)–based prediction to generate an extensive repertoire of structural models of human NUPs and their subcomplexes that cover various domains and interfaces that so far remained structurally uncharacterized and increases the structural coverage of the human NPC scaffold by about twofold.
References
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