Journal ArticleDOI
Enzyme-mediated oxidations for the chemist
TLDR
The present state of the art in the use of enzymes and microorganisms for catalytic oxidation and oxyfunctionalization chemistry is reviewed.About:
This article is published in Green Chemistry.The article was published on 2011-02-08. It has received 382 citations till now. The article focuses on the topics: Green chemistry & Catalytic oxidation.read more
Citations
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Biocatalysis: Enzymatic Synthesis for Industrial Applications
TL;DR: A review of biocatalysis with a special focus on scalable chemical production using enzymes discusses the opportunities and limitations of enzymatic syntheses using distinct examples and provides an outlook on emerging enzyme classes.
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Enzymatic reactions in confined environments
TL;DR: Some of the general principles of enzymatic reactions confined on surfaces, at interfaces, and inside small volumes are discussed and some of the necessary future steps to improve fundamental understanding of these systems are critically evaluated.
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Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently
TL;DR: Improving enzymes by directed evolution requires the navigation of very large search spaces; this work surveys how to do this intelligently.
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Enzymatic reductions for the chemist
TL;DR: In this contribution recent developments in the field of enzymatic reduction using whole cells and isolated enzymes are reviewed.
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Regio- and stereoselectivity of P450-catalysed hydroxylation of steroids controlled by laboratory evolution
TL;DR: It is demonstrated that directed evolution using iterative saturation mutagenesis provides a means to solve synthetic problems of this kind by preparing mutants that can regio- and stereoselectively oxidize either position.
References
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Journal ArticleDOI
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Dae-Kyun Ro,Eric M. Paradise,Mario Ouellet,Karl Fisher,Karyn L. Newman,John M. Ndungu,Ho Kimberly,Eachus Rachel,Timothy S. Ham,James Kirby,Michelle C. Y. Chang,Sydnor T. Withers,Yoichiro Shiba,Richmond Sarpong,Jay D. Keasling +14 more
TL;DR: The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
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Industrial biocatalysis today and tomorrow
TL;DR: Biocatalytic processes can now be carried out in organic solvents as well as aqueous environments, so that apolar organic compounds aswell as water-soluble compounds can be modified selectively and efficiently with enzymes and bioc atalytically active cells.
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Dioxygen activation at mononuclear nonheme iron active sites: enzymes, models, and intermediates.
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