Epidermal Growth Factor Receptor and Transforming Growth Factor-β Signaling Contributes to Variation for Wing Shape in Drosophila melanogaster
Ian Dworkin,Greg Gibson +1 more
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In this article, 50 insertional mutations, representing 43 loci in the RTK, Hedgehog, TGF-β pathways, and their genetically interacting factors were used to study the role of these networks on wing shape.Abstract:
Wing development in Drosophila is a common model system for the dissection of genetic networks and their roles during development. In particular, the RTK and TGF-β regulatory networks appear to be involved with numerous aspects of wing development, including patterning, cell determination, growth, proliferation, and survival in the developing imaginal wing disc. However, little is known as to how subtle changes in the function of these genes may contribute to quantitative variation for wing shape, per se. In this study 50 insertional mutations, representing 43 loci in the RTK, Hedgehog, TGF-β pathways, and their genetically interacting factors were used to study the role of these networks on wing shape. To concurrently examine how genetic background modulates the effects of the mutation, each insertion was introgressed into two wild-type genetic backgrounds. Using geometric morphometric methods, it is shown that the majority of these mutations have profound effects on shape but not size of the wing when measured as heterozygotes. To examine the relationships between how each mutation affects wing shape hierarchical clustering was used. Unlike previous observations of environmental canalization, these mutations did not generally increase within-line variation relative to their wild-type counterparts. These results provide an entry point into the genetics of wing shape and are discussed within the framework of the dissection of complex phenotypes.read more
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References
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Orna Cook,Brian Biehs,Ethan Bier +2 more
TL;DR: This study examines the mechanism by which two broadly expressed Dpp signaling target genes, optomotor-blind (omb) and brinker (brk), collaborate to initiate formation of the fifth longitudinal (L5) wing vein and shows that a border between omb and brk expression domains is necessary and sufficient for inducing L5 development in the posterior regions.
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Ability of Geometric Morphometric Methods to Estimate a Known Covariance Matrix
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Naturally occurring genetic variation affects Drosophila photoreceptor determination
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Association between nucleotide variation in Egfr and wing shape in Drosophila melanogaster.
Arnar Palsson,Greg Gibson +1 more
TL;DR: Whole-gene sequencing in very large samples, rather than selective genotyping, would appear to be the only strategy likely to be successful for detecting subtle associations in species with high polymorphism and little haplotypes, but features severely limit the ability of linkage disequilibrium mapping in Drosophila to resolve quantitative effects to single nucleotides.
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