Epidermal Growth Factor Receptor and Transforming Growth Factor-β Signaling Contributes to Variation for Wing Shape in Drosophila melanogaster
Ian Dworkin,Greg Gibson +1 more
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In this article, 50 insertional mutations, representing 43 loci in the RTK, Hedgehog, TGF-β pathways, and their genetically interacting factors were used to study the role of these networks on wing shape.Abstract:
Wing development in Drosophila is a common model system for the dissection of genetic networks and their roles during development. In particular, the RTK and TGF-β regulatory networks appear to be involved with numerous aspects of wing development, including patterning, cell determination, growth, proliferation, and survival in the developing imaginal wing disc. However, little is known as to how subtle changes in the function of these genes may contribute to quantitative variation for wing shape, per se. In this study 50 insertional mutations, representing 43 loci in the RTK, Hedgehog, TGF-β pathways, and their genetically interacting factors were used to study the role of these networks on wing shape. To concurrently examine how genetic background modulates the effects of the mutation, each insertion was introgressed into two wild-type genetic backgrounds. Using geometric morphometric methods, it is shown that the majority of these mutations have profound effects on shape but not size of the wing when measured as heterozygotes. To examine the relationships between how each mutation affects wing shape hierarchical clustering was used. Unlike previous observations of environmental canalization, these mutations did not generally increase within-line variation relative to their wild-type counterparts. These results provide an entry point into the genetics of wing shape and are discussed within the framework of the dissection of complex phenotypes.read more
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References
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Quantitative analysis of bristle number in Drosophila mutants identifies genes involved in neural development
Koenraad Norga,Marjorie C. Gurganus,Christy L. Dilda,Akihiko Yamamoto,Richard F. Lyman,Prajal H. Patel,Gerald M. Rubin,Roger A. Hoskins,Trudy F. C. Mackay,Hugo J. Bellen,Hugo J. Bellen +10 more
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Kenneth Weber,Robert Eisman,Lisa Morey,April Patty,Joshua Sparks,Michele Tausek,Zhao-Bang Zeng +6 more
TL;DR: Loci on the third chromosome of Drosophila melanogaster that affect an index of wing shape were mapped, using recombinant isogenic lines, with transposable elements as markers, and can be explained reasonably well by two models.
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Effects of Single P -Element Insertions on Olfactory Behavior in Drosophila melanogaster
TL;DR: Fourteen smell impaired (smi) lines were identified in which P[lArB] element insertion caused different degrees of hyposmia in one or both sexes, indicating that expression of at least 10 smi genes is controlled by olfactory tissue-specific promoter/enhancer elements.
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