Epidermal Growth Factor Receptor and Transforming Growth Factor-β Signaling Contributes to Variation for Wing Shape in Drosophila melanogaster
Ian Dworkin,Greg Gibson +1 more
Reads0
Chats0
TLDR
In this article, 50 insertional mutations, representing 43 loci in the RTK, Hedgehog, TGF-β pathways, and their genetically interacting factors were used to study the role of these networks on wing shape.Abstract:
Wing development in Drosophila is a common model system for the dissection of genetic networks and their roles during development. In particular, the RTK and TGF-β regulatory networks appear to be involved with numerous aspects of wing development, including patterning, cell determination, growth, proliferation, and survival in the developing imaginal wing disc. However, little is known as to how subtle changes in the function of these genes may contribute to quantitative variation for wing shape, per se. In this study 50 insertional mutations, representing 43 loci in the RTK, Hedgehog, TGF-β pathways, and their genetically interacting factors were used to study the role of these networks on wing shape. To concurrently examine how genetic background modulates the effects of the mutation, each insertion was introgressed into two wild-type genetic backgrounds. Using geometric morphometric methods, it is shown that the majority of these mutations have profound effects on shape but not size of the wing when measured as heterozygotes. To examine the relationships between how each mutation affects wing shape hierarchical clustering was used. Unlike previous observations of environmental canalization, these mutations did not generally increase within-line variation relative to their wild-type counterparts. These results provide an entry point into the genetics of wing shape and are discussed within the framework of the dissection of complex phenotypes.read more
Citations
More filters
Posted Content
Genetic Complexity in a Drosophila Model of Diabetes-Associated Misfolded Human Proinsulin
Soo-Young Park,Michael Ludwig,Natalia A. Tamarina,Bin Z. He,Sarah H. Carl,Desiree A. Dickerson,Levi Barse,Bharath Arun,Calvin L. Williams,Cecelia M. Miles,Louis H. Philipson,D. F. Steiner,Graeme I. Bell,Martin Kreitman +13 more
TL;DR: In this article, the authors used Drosophila melanogaster to create a genetic model of human permanent neonatal diabetes mellitus and present experimental results describing dimensions of this complexity, which involves the transgenic expression of a misfolded mutant of human preproinsulin, hINSC96Y, which is a cause of the disease.
Posted ContentDOI
Reexamining Waddington: Canalization and new mutations are not required for the evolution of genetic assimilation
TL;DR: It is demonstrated that a polygenic CGV, but not new variants of large effect are largely responsible for the evolved response demonstrated using both genomic and genetic approaches, and that CGV has potentially pleiotropic and fitness consequences in natural populations and may not be entirely “cryptic”.
Journal ArticleDOI
Cryptic genetic variation of eQTL architecture revealed by genetic perturbation in C. elegans.
Marijke H van Wijk,Joost A. G. Riksen,Mark Elvin,Gino B. Poulin,M. I. Maulana,Jan E. Kammenga,Basten L. Snoek,Mark G. Sterken +7 more
TL;DR: In this article , the authors investigated the change in transcriptional architecture of C. elegans and found evidence of accelerated transcriptional aging in the gld-1 RNAi treated nematodes.
RNA structures and their molecular evolution in HIV: evolution of robustness in RNA structures and theoretical systems
TL;DR: It is concluded that RNA structures in HIV do evolve via compensatory evolution, but that hypermutation can obscure the expected signal and pervasive environmental variation can select for environmental and genetic robustness in all three systems.
References
More filters
Journal ArticleDOI
R: A Language for Data Analysis and Graphics
Ross Ihaka,Robert Gentleman +1 more
TL;DR: In this article, the authors discuss their experience designing and implementing a statistical computing language, which combines what they felt were useful features from two existing computer languages, and they feel that the new language provides advantages in the areas of portability, computational efficiency, memory management, and scope.
Journal ArticleDOI
Extensions of the Procrustes Method for the Optimal Superimposition of Landmarks
F. James Rohlf,Dennis E. Slice +1 more
TL;DR: In this paper, a new method is presented that generalizes Siegel and Benson's (1982) resistant-fit theta-rho analysis so that more than two objects can be compared at the same time.
MonographDOI
Morphometric tools for landmark data : geometry and biology
TL;DR: In this article, the principal axes of shape change for triangles and features of shape comparison are discussed. But the authors do not discuss the relationship between landmarks and shape coordinates of triangles.
Book
Geometric morphometrics for biologists: a primer
TL;DR: The second edition of "Geometric Morphometrics for Biologists" represents the current state of the art and adds new examples and summarizes recent literature, as well as provides an overview of new software and step-by-step guidance through details of carrying out the analyses.
Journal ArticleDOI
Canalization of development and the inheritance of acquired characters
TL;DR: In this paper, the authors suggest that recent views on the nature of the developmental process make it easier to understand how the genotypes of evolving organisms can respond to the environment in a more co-ordinated fashion.
Related Papers (5)
The dimensionality of genetic variation for wing shape in Drosophila melanogaster
Jason G. Mezey,David Houle +1 more