Epigenetic activation of the MiR-200 family contributes to H19-mediated metastasis suppression in hepatocellular carcinoma
Ling Zhang,Fu Yang,Ji-hang Yuan,Sheng-xian Yuan,Weiping Zhou,Xi-song Huo,Dan Xu,Hai-shan Bi,Fang Wang,Shuhan Sun +9 more
TLDR
It is shown that H19 was underexpressed in intratumoral HCC tissues (T), as compared with peritumoral tissues (L), and low T/L ratio of H19 predicted poor prognosis, which could suggest the development of combination therapies that target H19 and the miR-200 family.Abstract:
Although numerous long non-coding RNAs (lncRNAs) have been identified in mammals, many of their biological roles remain to be characterized. Early reports suggest that H19 contributes to carcinogenesis, including hepatocellular carcinoma (HCC). Examination of the Oncomine resource showed that most HCC cases express H19 at a level that is comparable with the liver, with a tendency toward lower expression. This is consistent with our previous microarray data and indicates a more complicated role of H19 in HCC that needs to be characterized. In this study, the expression level of H19 was assessed in different regions of HCC patients' liver samples. Loss- and gain-of-function studies on this lncRNA in the HCC cell lines, SMMC7721 and HCCLM3, were used to characterize its effects on gene expression and to assess its effect on HCC metastasis both in vitro and in vivo. In this study, we show that H19 was underexpressed in intratumoral HCC tissues (T), as compared with peritumoral tissues (L). Additionally, low T/L ratio of H19 predicted poor prognosis. H19 suppressed HCC progression metastasis and the expression of markers of epithelial-to-mesenchymal transition. Furthermore, H19 associated with the protein complex hnRNP U/PCAF/RNAPol II, activating miR-200 family by increasing histone acetylation. The results demonstrate that H19 can alter the miR-200 pathway, thus contributing to mesenchymal-to-epithelial transition and to the suppression of tumor metastasis. These data provide an explanation for the hitherto puzzling literature on the relationship between H19 and cancer, and could suggest the development of combination therapies that target H19 and the miR-200 family.read more
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Baicalein inhibits breast cancer growth via activating a novel isoform of the long noncoding RNA PAX8-AS1-N.
TL;DR: Results demonstrated that PAX8‐AS1‐N activation mediated the anti‐cancer effects of baicalein via regulating miR‐17‐5p, and suggested that baicalsein and enhancing PAX8 •AS1 •N would be potential therapeutic strategies against breast cancer.
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Epigenetic Modifications in Thyroid Cancer Cells Restore NIS and Radio-Iodine Uptake and Promote Cell Death.
Sabine Wächter,Alexander I. Damanakis,Moritz Elxnat,Silvia Roth,Annette Wunderlich,Frederik A. Verburg,Sebastian A Fellinger,Detlef K. Bartsch,Pietro Di Fazio +8 more
TL;DR: Deacetylase inhibitors reduced cell viability, restored NIS and H19, and suppressed the oncogenes HMGA2 and TTF1 in thyroid cancer cells.
Journal ArticleDOI
lncRNA‐H19/miR‐29a axis affected the viability and apoptosis of keloid fibroblasts through acting upon COL1A1 signaling
TL;DR: H19 might facilitate proliferation and metastasis of fibroblasts by modifying downstream miR‐29a and COL1A1, which was expected to allow for development of keloid‐targeted treatments.
Journal ArticleDOI
Microarray expression profile analysis of long non-coding RNAs of advanced stage human gastric cardia adenocarcinoma
TL;DR: It is shown that lncRNAs dysregulation exerts important roles in human GCA lymph node metastasis, indicating that lNCRNAs are novel candidate biomarkers for the clinical diagnosis of advanced stage GCA and that could be targets for further therapy.
Journal ArticleDOI
Long non-coding RNA H19, a novel therapeutic target for pancreatic cancer.
TL;DR: It is suggested that H19 may be a novel therapeutic target for PDAC and the findings may open new revenues for scientific researches and development of valuable therapies for these diseases in the future.
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