Journal ArticleDOI
Epitope‐based immunoinformatics approach on RNA‐dependent RNA polymerase (RdRp) protein complex of Nipah virus (NiV)
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TLDR
The B‐cell epitope predictions suggest that the sequence positions 421 to 471 in phosphoprotein, 606 to 640 in polymerase and 496 to 517 in nucleocapsid protein are the best‐predicted regions for B‐ cell immune response, but further experimental circumstance is required to test and validate the efficacy of the subunit peptide for potential candidacy against NiV.Abstract:
Persistent outbreaks of Nipah virus (NiV) with severe case fatality throw a major challenge on researchers to develop a drug or vaccine to combat the disease. With little knowledge of its molecular mechanisms, we utilized the proteome data of NiV to evaluate the potency of three major proteins (phosphoprotein, polymerase, and nucleocapsid protein) in the RNA-dependent RNA polymerase complex to count as a possible candidate for epitope-based vaccine design. Profound computational analysis was used on the above proteins individually to explore the T-cell immune properties like antigenicity, immunogenicity, binding to major histocompatibility complex class I and class II alleles, conservancy, toxicity, and population coverage. Based on these predictions the peptide 'ELRSELIGY' of phosphoprotein and 'YPLLWSFAM' of nulceocapsid protein were identified as the best-predicted T-cell epitopes and molecular docking with human leukocyte antigen-C (HLA-C*12:03) molecule was effectuated followed by validation with molecular dynamics simulation. The B-cell epitope predictions suggest that the sequence positions 421 to 471 in phosphoprotein, 606 to 640 in polymerase and 496 to 517 in nucleocapsid protein are the best-predicted regions for B-cell immune response. However, the further experimental circumstance is required to test and validate the efficacy of the subunit peptide for potential candidacy against NiV.read more
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Journal ArticleDOI
Nipah virus: epidemiology, pathology, immunobiology and advances in diagnosis, vaccine designing and control strategies – a comprehensive review
Raj Kumar Singh,Kuldeep Dhama,Sandip Chakraborty,Ruchi Tiwari,Senthilkumar Natesan,Rekha Khandia,Ashok Munjal,Kranti Vora,Shyma K. Latheef,Kumaragurubaran Karthik,Yashpal Singh Malik,Rajendra Singh,Wanpen Chaicumpa,Devendra T. Mourya +13 more
TL;DR: Phylogenetic analysis affirmed the circulation of two major clades of Nipah virus as based on currently available complete N and G gene sequences, and high pathogenicity of NiV in humans, and lack of vaccines or therapeutics to counter this disease have attracted attention of researchers worldwide for developing effective NiV vaccine and treatment regimens.
Journal ArticleDOI
A promising antiviral candidate drug for the COVID-19 pandemic: A mini-review of remdesivir.
Liang Chengyuan,Lei Tian,Liu Yuzhi,Hui Nan,Qiao Guaiping,Li Han,Zhenfeng Shi,Tang Yonghong,Zhang Dezhu,Xie Xiaolin,Xu Zhao +10 more
TL;DR: This review presents comprehensive information on remdesivir, including information regarding the milestones, intellectual properties, anti-coronavirus mechanisms, preclinical research and clinical trials, and in particular, the chemical synthesis, pharmacology, toxicology, pharmacodynamics and pharmacokinetics of remdesvir.
Journal ArticleDOI
Reverse vaccinology approach to design a novel multi-epitope subunit vaccine against avian influenza A (H7N9) virus.
Mahmudul Hasan,Progga paromita Ghosh,Kazi Faizul Azim,Shamsunnahar Mukta,Ruhshan Ahmed Abir,Jannatun Nahar,Mohammad Mehedi Hasan Khan +6 more
TL;DR: An in silico reverse vaccinology strategy was adopted to design a unique chimeric subunit vaccine against avian influenza A (H7N9), designed by the combination of highly immunogenic epitopes along with suitable adjuvant and linkers.
Journal ArticleDOI
Strategic Development of a Next-Generation Multi-Epitope Vaccine To Prevent Nipah Virus Zoonotic Infection
TL;DR: The next-generation approach will provide a new vision for the development of a high immunogenic vaccine against the NiV and generate the humoral as well as cell-mediated immunity.
Journal ArticleDOI
Extensive immunoinformatics study for the prediction of novel peptide-based epitope vaccine with docking confirmation against envelope protein of Chikungunya virus: a computational biology approach
Syed Shahariar Bappy,Sorna Sultana,Juthi Adhikari,Shafi Mahmud,Md. Arif Khan,K. M. Kaderi Kibria,Md. Masuder Rahman,Abu Zaffar Shibly +7 more
TL;DR: Chikungunya virus (CHIKV) instigating Chikunguna fever is a global infective menace resulting in high fever, weakened joint-muscle pain, and brain inflammation as discussed by the authors.
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