Journal ArticleDOI
Estrogen and Progesterone Control of Gene Expression in the Mouse Meibomian Gland
TLDR
The findings show that 17beta-estradiol, progesterone, or both hormones administered together significantly influenced the expression of numerous genes in the mouse meibomian gland.Abstract:
PURPOSE. The purpose of the study was to test the hypothesis that estrogen and progesterone regulate gene expression in the meibomian gland. METHODS. Meibomian glands were obtained from young adult, ovariectomized mice that were administered 17-estradiol, progesterone, 17-estradiol plus progesterone, or vehicle for 14 days. Glands were pooled according to treatment, processed for the extraction of RNA, and analyzed for differentially expressed mRNAs by using mouse gene microarrays. Bioarray data were evaluated with sophisticated bioinformatics software and statistical programs. The expression of selected genes was confirmed with gene chips and quantitative realtime PCR techniques. RESULTS. The findings show that 17-estradiol, progesterone, or both hormones administered together significantly influenced the expression of numerous genes in the mouse meibomian gland. Notable were the effects of 17-estradiol on genes related to lipid metabolism, tyrosine kinases, immune factors, extracellular matrix components, steroidogenesis, and prolactin dynamics. Also very significant were the actions of progesterone or 17-estradiol plus progesterone on ribosome or localization gene ontologies, respectively. The various hormone treatments led to many analogous, opposite, or unique effects on gene expression. CONCLUSIONS. These findings support the study hypothesis that estrogen and progesterone modulate gene expression in the meibomian gland. (Invest Ophthalmol Vis Sci. 2008;49: 1797‐1808) DOI:10.1167/iovs.07-1458read more
Citations
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The International Workshop on Meibomian Gland Dysfunction: Report of the Subcommittee on Anatomy, Physiology, and Pathophysiology of the Meibomian Gland
TL;DR: It is accepted that MGD is important, conceivably underestimated, and possibly the most frequent cause of dry eye disease due to increased evaporation of the aqueous tears, and a comprehensive review of physiological and pathophysiological aspects of the meibomian glands is sought.
Journal ArticleDOI
TFOS DEWS II Sex, Gender, and Hormones Report
David A. Sullivan,Eduardo Rocha,Pasquale Aragona,Janine A. Clayton,Juan Ding,Blanka Golebiowski,Ulrike Hampel,Alison M. McDermott,Debra A. Schaumberg,Debra A. Schaumberg,Sruthi Srinivasan,Piera Versura,Mark D. P. Willcox +12 more
TL;DR: Overall, sex, gender and hormones play a major role in the regulation of ocular surface and adnexal tissues, and in the difference in DED prevalence between women and men.
Journal ArticleDOI
Human tear film and meibum. Very long chain wax esters and (O-acyl)-omega-hydroxy fatty acids of meibum
TL;DR: Due to their amphiphilic anionogenic nature, OAHFAs may be responsible for stabilization of the tear film lipid layer by creating an interface between the vast pool of strictly nonpolar lipids of meibum (WEs, cholesteryl esters, etc.) and the aqueous subphase beneath it, a role previously attributed to phospholipids.
Journal ArticleDOI
The Meibomian puzzle: combining pieces together.
TL;DR: The most intriguing development is the virtual absence in meibum of typical phospholipids - an important group of amphiphilic compounds whose role in the human tear film was thought to be to stabilize the entire tear film structure.
Journal Article
Do sex steroids exert sex-specific and/or opposite effects on gene expression in lacrimal and meibomian glands?
TL;DR: The results support the hypothesis that sex steroids may induce sex-specific and/or opposite effects in the lacrimal and meibomian glands and whether these actions contribute to the prevalence of dry eye remains to be determined.
References
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