Journal ArticleDOI
Evaluation of the micromethod for determination of glutathione using enzymatic cycling and Ellman's reagent
Peter Eyer,Dušan Podhradský +1 more
Reads0
Chats0
TLDR
Evaluation of the kinetic parameters of the various reactions involved in the determination of glutathione provided the rationale for a modification of the frequently used assay whereby the enzymatic reaction is no longer rate limiting.About:
This article is published in Analytical Biochemistry.The article was published on 1986-02-15. It has received 185 citations till now. The article focuses on the topics: Ellman's reagent & Glutathione reductase.read more
Citations
More filters
Journal ArticleDOI
Assay of glutathione reductase in crude tissue homogenates using 5,5'-dithiobis(2-nitrobenzoic acid).
TL;DR: The specificity of DTNB allows assay of glutathione reductase in the presence of other NADPH-dependent enzymes and common protein extract contaminants.
Journal ArticleDOI
Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples.
TL;DR: By combining the least complicated and expedient methods of sample handling with the sensitivity and specificity of the GSH assay by enzymatic recycling and the small volumes and software capabilities of microtiter plate technology, this work devised a rapid, sensitive, and easy assay for GSH and GSSG in biological samples.
Journal ArticleDOI
Human hsp27, Drosophila hsp27 and human alphaB-crystallin expression-mediated increase in glutathione is essential for the protective activity of these proteins against TNFalpha-induced cell death
TL;DR: The results suggest that the protective activity shared by human hsp27, Drosophila hsp 27 and human alphaB‐crystallin against TNFalpha‐mediated cell death and probably other types of oxidative stress results from their conserved ability to raise the intracellular concentration of glutathione.
Journal ArticleDOI
Evidence that mitochondrial respiration is a source of potentially toxic oxygen free radicals in intact rabbit hearts subjected to ischemia and reflow.
Giuseppe Ambrosio,Jay L. Zweier,Carlo Duilio,Periannan Kuppusamy,Giuseppe E. Santoro,P. P. Elia,Isabella Tritto,Plinio Cirillo,Mario Condorelli,Massimo Chiariello +9 more
TL;DR: The hypothesis that resumption of mitochondrial respiration upon reperfusion might be a mechanism of oxygen radical formation in postischemic hearts, and that treatment with inhibitors of mitochondrialrespiration might prevent this phenomenon is tested, is tested.
References
More filters
Journal ArticleDOI
Tissue sulfhydryl groups
TL;DR: A water-soluble (at pH 8) aromatic disulfide [5,5′-dithiobis(2-nitrobenzoic acid] has been synthesized and shown to be useful for determination of sulfhydryl groups.
Journal ArticleDOI
Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues
TL;DR: The use of the foregoing analytical method in the determination of total and oxidized glutathione contents of rat blood, kidney, and liver gave values in good agreement with those obtained by previous investigators.
Journal ArticleDOI
Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine
TL;DR: It is reported here that 2-vinylpyridine is a much better reagent for the derivitization of glutathione, and it is demonstrated that the total glutATHione concentration in mouse plasma is substantially higher than generally reported and that glutathion disulfide constitutes less than 30% of the totalglutathione present.
Journal ArticleDOI
Purification and characterization of the flavoenzyme glutathione reductase from rat liver.
Inger Carlberg,Bengt Mannervik +1 more
TL;DR: The steady state kinetic data are consistent with a branching reaction mechanism previously proposed for glutathione reductase from yeast, and at low GSSG concentrations the rate equation can be approximated by that of a simple ping pong mechanism.
Journal ArticleDOI
A colorimetric method for determining low concentrations of mercaptans.
TL;DR: A study of the specificity of the reaction indicates that it occurs only with the S − group (actual or potential, as in thiourea), and heavy-metal ions interfere with the reaction by irreversibly bonding any S − ions.