Evaluation of three extraction-free SARS-CoV-2 RT-PCR assays: A feasible alternative approach with low technical requirements.
Benoit Visseaux,Gilles Collin,Nadhira Houhou-Fidouh,Quentin Le Hingrat,Valentine Marie Ferré,Florence Damond,Houria Ichou,Diane Descamps,Charlotte Charpentier +8 more
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TLDR
In this paper, three extraction-free RT-PCR assays were assessed: (i) PrimeDirect® Probe RT-qPCR Mix (Takara), (ii) PrimeScript® RT-QPCR (Tsara), and (iii) SARS-CoV-2 SANSURE®BIOTECH Novel Coronavirus (Sansure).About:
This article is published in Journal of Virological Methods.The article was published on 2021-05-01 and is currently open access. It has received 15 citations till now.read more
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RNA reference materials with defined viral RNA loads of SARS-CoV-2—A useful tool towards a better PCR assay harmonization
Laura Vierbaum,Nathalie Wojtalewicz,Hans-Peter Grunert,Vanessa Lindig,Ulf Duehring,Christian Drosten,Victor M. Corman,Daniela Niemeyer,Sandra Ciesek,Holger F. Rabenau,Annemarie Berger,Martin Obermeier,Andreas Nitsche,Janine Michel,Martin Mielke,Jim F. Huggett,Denise M. O'Sullivan,Eloise J. Busby,Simon Cowen,Peter M. Vallone,Megan H. Cleveland,Samreen Falak,A. Kummrow,Thomas Keller,Ingo Schellenberg,Heinz Zeichhardt,Martin Kammel +26 more
TL;DR: It is demonstrated that an indispensable prerequisite for linking Ct values to SARS-CoV-2 viral loads is that they are treated as being unique to an individual laboratory, and clinical guidance based on viral loads should not cite Ct values.
Journal ArticleDOI
Evaluation of extraction-free RT-qPCR methods for SARS-CoV-2 diagnostics.
Alexander Domnich,Vanessa De Pace,Beatrice Marina Pennati,Patrizia Caligiuri,Serena Varesano,Bianca Bruzzone,Andrea Orsi +6 more
TL;DR: In this paper, the authors compared the sensitivity of traditional extraction-based, heated extraction-free, and unheated extraction free RT-qPCR methods for SARS-CoV-2 detection in nasopharyngeal swabs from symptomatic individuals.
Journal ArticleDOI
Recent findings and applications of biomedical engineering for COVID-19 diagnosis: a critical review.
Le Minh Bui,Huong Thi Thu Phung,Thuy-Tien Ho Thi,Vijai Singh,Rupesh Maurya,Khushal Khambhati,Chia-Ching Wu,Jamal Uddin,Do Minh Trung,Dinh-Toi Chu,Dinh-Toi Chu +10 more
TL;DR: In this article, a review of the most recent diagnostic approaches and their potential in the context of the COVID-19 pandemic has been presented, highlighting the potential of each research direction.
Journal ArticleDOI
Current diagnostic approaches to detect two important betacoronaviruses: Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Zhi Xiong Chong,Winnie Pui Pui Liew,Hui Kian Ong,Chean Yeah Yong,Chong Seng Shit,Wan Yong Ho,Stephanie Y. L. Ng,Swee Keong Yeap +7 more
TL;DR: In this paper, the authors provide an up-to-date comparison of the advantages and limitations of different diagnostic approaches in detecting MERS and SARS-CoV-2 infections.
Journal ArticleDOI
Temporal stability and detection sensitivity of the dry swab-based diagnosis of SARS-CoV-2
C G Gokulan,Uday Kiran,Uday Kiran,Santosh Kumar Kuncha,Santosh Kumar Kuncha,Rakesh Mishra,Rakesh Mishra +6 more
TL;DR: In this paper, the temporal stability and performance of the dry swab viral detection method at two different temperatures were compared with standard VTM-RNA based method, and the results collectively suggest that dry swabs samples are stable at RT for 24 h and the detection of SARS-CoV-2 RNA by RT-PCR do not show variance from VTM RNA.
References
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Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
Ioanna Smyrlaki,Martin Ekman,Antonio Lentini,Nuno Rufino de Sousa,Natali Papanicolaou,Martin Vondracek,Johan Aarum,Hamzah Safari,Shaman Muradrasoli,Antonio Gigliotti Rothfuchs,Jan Albert,Jan Albert,Björn Högberg,Björn Reinius +13 more
TL;DR: Methods circumventing RNA extraction in COVID-19 testing by performing RT-PCR directly on heat-inactivated or lysed samples are described and significant savings in time and cost are achieved through RNA-extraction-free protocols that are directly compatible with established PCR-based testing pipelines.
Journal ArticleDOI
Rapid isothermal amplification and portable detection system for SARS-CoV-2.
Anurup Ganguli,Ariana Mostafa,Jacob Berger,Mehmet Y. Aydin,Fu Sun,Sarah A Stewart de Ramirez,Enrique Valera,Brian T. Cunningham,William P. King,William P. King,Rashid Bashir,Rashid Bashir +11 more
TL;DR: An RT-LAMP isothermal assay for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus is reported and demonstrated on clinical samples using a simple and accessible point-of-care (POC) instrument.
Journal ArticleDOI
Viral cultures for COVID-19 infectious potential assessment - a systematic review.
TL;DR: The data suggest a relationship between the time from onset of symptom to the timing of the specimen test, cycle threshold (Ct) and symptom severity, which should be used to define the reliability of PCR for assessing infectious potential.
Journal ArticleDOI
Strategies and perspectives to develop SARS-CoV-2 detection methods and diagnostics.
Rekha Jalandra,Amit K. Yadav,Damini Verma,Nishu Dalal,Minakshi Sharma,Rajeev Singh,Anil Kumar,Pratima R. Solanki +7 more
TL;DR: This review will discuss multiple detection methods for coronavirus for developing appropriate, reliable, and fast alternative detection methods.
Journal ArticleDOI
Extraction-free SARS-CoV-2 detection by rapid RT-qPCR universal for all primary respiratory materials.
Nadine Lübke,Tina Senff,Sara Scherger,Sandra Hauka,Marcel Andree,Ortwin Adams,Jörg Timm,Andreas Walker +7 more
TL;DR: This protocol for the detection of SARS-CoV-2 in crude material using a prepared frozen-PCR mix with optimized primers and 5 µl of fresh, undiluted and pre-analytically heat inactivated respiratory material is fast, inexpensive and suitable for all respiratory materials.