Open AccessJournal Article
Excision of the Drosophila Mariner Transposon Mos1: Comparison with Bacterial Transposition and V(D)J Recombination.: Comparison with Bacterial Transposition and V(D)J Recombination.
Angela Dawson,David J. Finnegan +1 more
TLDR
The mechanism by which mariner, a eukaryotic transposable element, performs DNA cleavage is examined and it is shown that the nontransferred strand is cleaved initially, unlike prokaryotictransposons which cleave the transferred strand first.About:
This article is published in Molecular Cell.The article was published on 2003-01-01 and is currently open access. It has received 80 citations till now. The article focuses on the topics: Transposition (music) & V(D)J recombination.read more
Citations
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Journal ArticleDOI
DNA Transposons and the Evolution of Eukaryotic Genomes
TL;DR: This review focuses on DNA-mediated or class 2 transposons and emphasizes how this class of elements is distinguished from other types of mobile elements in terms of their structure, amplification dynamics, and genomic effect.
Journal ArticleDOI
The outs and ins of transposition: from Mu to Kangaroo
TL;DR: Transposons are ubiquitous in prokaryotic and eukaryotic organisms and are major determinants of genome structure.
Journal ArticleDOI
Everyman's Guide to Bacterial Insertion Sequences
TL;DR: This review presents an overview of the current understanding of these transposable elements (TE), their organization and their transposition mechanism as well as their distribution and genomic impact, and provides a detailed description of the expanding variety of IS.
Journal ArticleDOI
Targeted engineering of the Caenorhabditis elegans genome following Mos1‐triggered chromosomal breaks
TL;DR: The Drosophila element Mos1 is a class II transposon, which moves by a ‘cut‐and‐paste’ mechanism and can be experimentally mobilized in the Caenorhabditis elegans germ line, and this pathway was further characterized to develop an efficient tool—called MosTIC—to manipulate the C. elegans genome.
Journal ArticleDOI
Molecular Architecture of the Mos1 Paired-End Complex: The Structural Basis of DNA Transposition in a Eukaryote
TL;DR: Crystallographic analyses of the paired-end complex (PEC) formed from precleaved transposon ends and the transposase of the eukaryotic element Mos1 reveals two parallel ends bound to a dimeric enzyme.
References
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Journal ArticleDOI
RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination.
TL;DR: The vast repertoire of immunoglobulins and T cell receptors is generated, in part, by V(D)J recombination, a series of genomic rearrangements that occur specifically in developing lymphocytes.
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Hairpin Opening and Overhang Processing by an Artemis/DNA-Dependent Protein Kinase Complex in Nonhomologous End Joining and V(D)J Recombination
TL;DR: Artemis forms a complex with the 469 kDa DNA-dependent protein kinase (DNA-PKcs) in the absence of DNA to permit enzymatic activities that are critical for the hairpin-opening step of V(D)J recombination and for the 5' and 3' overhang processing in nonhomologous DNA end joining.
Journal ArticleDOI
DNA Transposons and the Evolution of Eukaryotic Genomes
TL;DR: This review focuses on DNA-mediated or class 2 transposons and emphasizes how this class of elements is distinguished from other types of mobile elements in terms of their structure, amplification dynamics, and genomic effect.
Journal ArticleDOI
Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system
TL;DR: The results support the theory that RAG1 and RAG2 were once components of a transposable element, and that the split nature of immunoglobulin and T-cell-receptor genes derives from germline insertion of this element into an ancestral receptor gene soon after the evolutionary divergence of jawed and jawless vertebrates.
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V(D)J Recombination: RAG Proteins, Repair Factors, and Regulation*
TL;DR: V(D)J recombination is strongly regulated by limiting access to RSS sites within chromatin, so that particular sites are available only in certain cell types and developmental stages, and the roles of enhancers, histone acetylation, and chromatin remodeling factors in controlling accessibility are discussed.
Related Papers (5)
Tn7 transposition creates a hotspot for homologous recombination at the transposon donor site.
Ann T. Hagemann,Nancy L. Craig +1 more
Mutant Mos1 mariner transposons are hyperactive in Aedes aegypti.
David W. Pledger,Craig J. Coates +1 more