Journal ArticleDOI
Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale
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DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions.Abstract:
DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration. The expression profiles observed for genes with known metabolic functions pointed to features of the metabolic reprogramming that occur during the diauxic shift, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions. The same DNA microarrays were also used to identify genes whose expression was affected by deletion of the transcriptional co-repressor TUP1 or overexpression of the transcriptional activator YAP1. These results demonstrate the feasibility and utility of this approach to genomewide exploration of gene expression patterns.read more
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Principles of Gene Manipulation and Genomics
TL;DR: The principles of gene manipulation and genomics and their applications in medicine, science and technology are studied.
Journal ArticleDOI
HAP-Like CCAAT-binding complexes in filamentous fungi: implications for biotechnology.
Axel A. Brakhage,Alex Andrianopoulos,Masashi Kato,Stefan Steidl,Meryl A. Davis,Norihiro Tsukagoshi,Michael J. Hynes +6 more
TL;DR: The recent cloning of genes encoding the components of the corresponding complex (AnCF/PENR1) of Aspergillus nidulans and characterization of CCAAT-regulated genes in A. ndulans led to a deeper insight into the role of this transcription complex, in particular in aerobically growing fungi.
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Reconstruction of the yeast Snf1 kinase regulatory network reveals its role as a global energy regulator
Renata Usaite,Renata Usaite,Michael C. Jewett,Ana Paula Oliveira,John R. Yates,Lisbeth Olsson,Jens Nielsen +6 more
TL;DR: The findings showed that Snf1 is a low‐energy checkpoint and that yeast can be used more extensively as a model system for studying the molecular mechanisms underlying the global regulation of AMPK in mammals, failure of which leads to metabolic diseases.
Journal Article
Gene Expression After Treatment With Hydrogen Peroxide, Menadione, or T-Butyl Hydroperoxide in Breast Cancer Cells
Yao-Yu Eric Chuang,Yi Chen,Gadisetti,V R Chandramouli,John A. Cook,Deborah Coffin,Mong-Hsun Tsai,William DeGraff,Hailing Yan,Shuping Zhao,Angelo Russo,Edison T. Liu,James B. Mitchell +12 more
TL;DR: Interestingly, the patterns of gene induction were highly similar among the three oxidants; however, differences were observed, particularly with respect to p53, IL-6, and heat-shock related genes.
Journal ArticleDOI
Identifying phase-specific genes in the fungal pathogen Histoplasma capsulatum using a genomic shotgun microarray.
TL;DR: Genes induced in the mycelial phase included several involved in conidiation, cell polarity, and melanin production in other organisms, extending previous observations that sulfur metabolism influences morphology in H. capsulatum.
References
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Journal ArticleDOI
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TL;DR: A high-capacity system was developed to monitor the expression of many genes in parallel by means of simultaneous, two-color fluorescence hybridization, which enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA.
PatentDOI
Expression monitoring by hybridization to high density oligonucleotide arrays
TL;DR: In this article, the authors proposed a method for monitoring the expression levels of a multiplicity of genes by hybridizing a nucleic acid sample to a high density array of oligonucleotide probes and quantifying the hybridized nucleic acids in the array.
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Use of a cDNA microarray to analyse gene expression patterns in human cancer.
Joseph L. DeRisi,Lolita Penland,Patrick O. Brown,M. L. Bittner,P. S. Meltzer,M. Ray,Yi Chen,Y. A. Su,J. M. Trent +8 more
TL;DR: Previously unrecognized alterations in the expression of specific genes provide leads for further investigation of the genetic basis of the tumorigenic phenotype of these cells.
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Accessing Genetic Information with High-Density DNA Arrays
Mark S. Chee,Robert Yang,Earl Hubbell,Anthony Berno,Xiaohua C. Huang,David Stern,Jim Winkler,David J. Lockhart,Macdonald S. Morris,Stephen P. A. Fodor +9 more
TL;DR: The simultaneous analysis of the entire human mitochondrial genome is described here and can be used to address a variety of questions in molecular genetics including gene expression, genetic linkage, and genetic variability.
Journal ArticleDOI
A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization.
TL;DR: This work describes a general experimental approach, using microscopic arrays of DNA fragments on glass substrates for differential hybridization analysis of fluorescently labeled DNA samples, and demonstrates the utility of DNA microarrays in the analysis of complex DNA samples.