Journal ArticleDOI
Expression of engrailed proteins in arthropods, annelids and chordates
TLDR
A monoclonal antibody is described that recognizes a conserved epitope in the homeodomain of engrailed proteins of a number of different arthropods, annelids, and chordates; this antibody is used to isolate the grasshopperEngrailed gene, a homeobox gene that has an important role in Drosophila segmentation.About:
This article is published in Trends in Genetics.The article was published on 1989-01-01. It has received 582 citations till now. The article focuses on the topics: engrailed.read more
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Avian hairy Gene Expression Identifies a Molecular Clock Linked to Vertebrate Segmentation and Somitogenesis
TL;DR: The identified and characterized c-hairy1, an avian homolog of the Drosophila segmentation gene, hairy, provides molecular evidence for a developmental clock linked to segmentation and somitogenesis of the paraxial mesoderm and support the possibility that segmentation mechanisms used by invertebrates and vertebrates have been conserved.
Journal ArticleDOI
Organizing activity of wingless protein in Drosophila.
Gary Struhl,Konrad Basler +1 more
TL;DR: It is proposed that wg protein organizes leg pattern along the dorsoventral axis by conferring ventral positional information within the disc.
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A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary
Haifan Lin,Allan C. Spradling +1 more
TL;DR: The self-renewing asymmetric division of germline stem cells in the Drosophila ovarian germline, as marked by the spectrosome, a cytoplasmic structure rich in membrane skeletal proteins that marks the lineage of germ line stem cells is described.
Journal ArticleDOI
Proteolysis that is inhibited by hedgehog targets Cubitus interruptus protein to the nucleus and converts it to a repressor.
Pedro Aza-Blanc,Felipe-Andrés Ramírez-Weber,Marie-Pierre Laget,Carol Schwartz,Thomas B. Kornberg +4 more
TL;DR: It is reported here the identification of a domain that tethers Ci in the cytoplasm and show that in some anterior cells, Ci is cleaved to generate a form that lacks the tethering domain and this form translocates to the nucleus where it represses hh and other target genes.
Journal ArticleDOI
The protein product of the zebrafish homologue of the mouse T gene is expressed in nuclei of the germ ring and the notochord of the early embryo.
TL;DR: The cloning and sequencing of the T gene in the zebrafish is reported and the nuclear localization of the protein product is shown, indicating high levels of expression are maintained throughout early development in the notochord.
References
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Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
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Continuous cultures of fused cells secreting antibody of predefined specificity
G Köhler,C. Milstein +1 more
TL;DR: The derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies is described here, made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor.
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A series of normal stages in the development of the chick embryo
TL;DR: In this article, a series of normal stages of the chick embryo is described in terms of the length of time of incubation, except for the first three days during which more detailed characteristics such as the number of somites are applied.
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Mutations affecting segment number and polarity in Drosophila
TL;DR: The phenotypes of the mutant embryos indicate that the process of segmentation involves at least three levels of spatial organization: the entire egg as developmental unit, a repeat unit with the length of two segments, and the individual segment.