Fabrication of Microarray of Gel-Immobilized Compounds on a Chip by Copolymerization
A.V. Vasiliskov,Edward N. Timofeev,Edward N. Timofeev,Sergey Surzhikov,Sergey Surzhikov,A. L. Drobyshev,A. L. Drobyshev,A. L. Drobyshev,V.V. Shick,V.V. Shick,Andrei D. Mirzabekov,Andrei D. Mirzabekov,Andrei D. Mirzabekov +12 more
TLDR
The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 x 10 to 100 x 100 microns, is described.Abstract:
The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 × 10 to 100 × 100 μm, is described. The microchips are produced by phot...read more
Citations
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Multifunctional encoded particles for high-throughput biomolecule analysis.
TL;DR: This work presents a method based on continuous-flow lithography that combines particle synthesis and encoding and probe incorporation into a single process to generate multifunctional particles bearing over a million unique codes that can be scanned rapidly in a flow-through microfluidic channel.
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Protein microchips: use for immunoassay and enzymatic reactions.
Pavel Arenkov,Alexander Kukhtin,Anne Gemmell,Sergey Voloshchuk,Valentina Vladimirovna Chupeeva,Andrei D. Mirzabekov,Andrei D. Mirzabekov +6 more
TL;DR: Protein microchips were used in immunoassays for detection of antigens or antibodies, as well as to carry out enzymatic reactions and to measure their kinetics in the absence or presence of an inhibitor.
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Molecular profiling of human cancer.
TL;DR: Traditional approaches to cancer classification and diagnosis have been based on histological examination, but studies have uncovered classes of cancer that extend traditional classification on the basis of histology and morphology, such as metastasis.
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Fluorescent in situ sequencing on polymerase colonies
TL;DR: Four advances that bring us closer to sequencing genomes cost-effectively using the polony technology are presented and a polymerase trapping technique enables efficient nucleotide extension by DNA polymerase in a polyacrylamide matrix and eliminates loss of enzyme during sequencing cycles.
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Solid supports for microarray immunoassays
Wlad Kusnezow,Jörg D. Hoheisel +1 more
TL;DR: The sensitivity of microspot immunoassays predicted by the current analyte theory is not yet a reality, indicating the extent of both the technology's potential and the size of the task still ahead.
References
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Expression monitoring by hybridization to high density oligonucleotide arrays
TL;DR: In this article, the authors proposed a method for monitoring the expression levels of a multiplicity of genes by hybridizing a nucleic acid sample to a high density array of oligonucleotide probes and quantifying the hybridized nucleic acids in the array.
Journal ArticleDOI
Light-directed, spatially addressable parallel chemical synthesis.
TL;DR: High-density arrays formed by light-directed synthesis are potentially rich sources of chemical diversity for discovering new ligands that bind to biological receptors and for elucidating principles governing molecular interactions.
Journal ArticleDOI
Detection of heterozygous mutations in BRCA1 using high density oligonucleotide arrays and two-colour fluorescence analysis.
TL;DR: Fourteen of fifteen patient samples with known mutations were accurately diagnosed, and no false positive mutations were identified in 20 control samples, suggesting DNA chip–based assays may provide a valuable new technology for high–throughput cost–efficient detection of genetic alterations.
Journal ArticleDOI
Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ.
Uwe Maskos,Edwin M. Southern +1 more
TL;DR: A novel linker for the synthesis of oligonucleotides on a glass support remains tethered to the support after ammonia treatment and are shown to take part in sequence specific hybridisation reactions.
Journal ArticleDOI
Rapid determination of single base mismatch mutations in DNA hybrids by direct electric field control
TL;DR: It is demonstrated that controlled electric fields can be used to regulate transport, concentration, hybridization, and denaturation of single- and double-stranded oligonucleotides and single base pair mismatch discrimination is carried out rapidly and with high resolution.