Journal ArticleDOI
Printing proteins as microarrays for high-throughput function determination.
TLDR
Miniaturized assays that accommodate extremely low sample volumes and enable the rapid, simultaneous processing of thousands of proteins are developed to facilitate subsequent studies of protein function.Abstract:
Systematic efforts are currently under way to construct defined sets of cloned genes for high-throughput expression and purification of recombinant proteins To facilitate subsequent studies of protein function, we have developed miniaturized assays that accommodate extremely low sample volumes and enable the rapid, simultaneous processing of thousands of proteins A high-precision robot designed to manufacture complementary DNA microarrays was used to spot proteins onto chemically derivatized glass slides at extremely high spatial densities The proteins attached covalently to the slide surface yet retained their ability to interact specifically with other proteins, or with small molecules, in solution Three applications for protein microarrays were demonstrated: screening for protein-protein interactions, identifying the substrates of protein kinases, and identifying the protein targets of small moleculesread more
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Nanostructures in Biodiagnostics
Nathaniel L. Rosi,Chad A. Mirkin +1 more
TL;DR: Nathaniel L. Rosi focuses on the rational assembly of DNA-modified nanostructures into larger-scale materials and their roles in biodiagnostic screening for nucleic acids.
Journal ArticleDOI
A comprehensive two-hybrid analysis to explore the yeast protein interactome
TL;DR: The comprehensive analysis using a system to examine two-hybrid interactions in all possible combinations between the budding yeast Saccharomyces cerevisiae is completed and would significantly expand and improve the protein interaction map for the exploration of genome functions that eventually leads to thorough understanding of the cell as a molecular system.
Journal ArticleDOI
Understanding plant responses to drought — from genes to the whole plant
TL;DR: Attention is drawn to the perception and signalling processes (chemical and hydraulic) of water deficits, which are essential for a holistic understanding of plant resistance to stress, which is needed to improve crop management and breeding techniques.
Journal ArticleDOI
Designing materials for biology and medicine
Robert Langer,David A. Tirrell +1 more
TL;DR: New challenges and directions in biomaterials research are discussed, including synthetic replacements for biological tissues, designing materials for specific medical applications, and materials for new applications such as diagnostics and array technologies.
Journal ArticleDOI
Anisotropic metal nanoparticles: Synthesis, assembly, and optical applications
Catherine J. Murphy,Tapan K. Sau,Anand Gole,Christopher J. Orendorff,Jinxin Gao,Linfeng Gou,Simona E. Hunyadi,Tan Li +7 more
TL;DR: Variations in reaction conditions and crystallographic analysis of gold nanorod have led to insight into the growth mechanism of these materials, and optical applications in sensing and imaging, which take advantage of the visible light absorption and scattering properties of the nanorods are discussed.
References
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Journal ArticleDOI
Quantitative monitoring of gene expression patterns with a complementary DNA microarray.
TL;DR: A high-capacity system was developed to monitor the expression of many genes in parallel by means of simultaneous, two-color fluorescence hybridization, which enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA.
Journal ArticleDOI
The capsaicin receptor: a heat-activated ion channel in the pain pathway
Michael J. Caterina,Mark Schumacher,Makoto Tominaga,Tobias A. Rosen,Jon D. Levine,David Julius +5 more
TL;DR: The cloned capsaicin receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo.
Journal ArticleDOI
A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae
Peter Uetz,Loic Giot,Gerard Cagney,Traci A. Mansfield,Richard S. Judson,James R. Knight,Daniel Lockshon,Vaibhav A. Narayan,Maithreyan Srinivasan,Pascale Pochart,Alia Qureshi-Emili,Ying Li,Brian C. Godwin,Diana Conover,Theodore S. Kalbfleisch,Govindan Vijayadamodar,Meijia Yang,Mark Johnston,Stanley Fields,Jonathan M. Rothberg +19 more
TL;DR: Examination of large-scale yeast two-hybrid screens reveals interactions that place functionally unclassified proteins in a biological context, interactions between proteins involved in the same biological function, and interactions that link biological functions together into larger cellular processes.
Journal ArticleDOI
I kappa B: a specific inhibitor of the NF-kappa B transcription factor.
TL;DR: The data show the existence of a phorbol ester-responsive regulatory protein that acts by controlling the DNA binding activity and subcellular localization of a transcription factor in cells that do not express immunoglobulin kappa light chain genes.
Journal ArticleDOI
A mammalian protein targeted by G1-arresting rapamycin–receptor complex
Eric J. Brown,Mark W. Albers,Tae Bum Shin,Kazuo ichikawa,Curtis Keith,William S. Lane,Stuart L. Schreiber +6 more
TL;DR: A mammalian FKBP–rapamycin-associated protein (FRAP) is isolate whose binding to structural variants of rapamycin complexed to FK BP12 correlates with the ability of these ligands to inhibit cell-cycle progression.