Journal ArticleDOI
Factors responsible for ADP-induced release reaction of human platelets
TLDR
Although fibrinogen is required for ADP-induced primary aggregation, it does not support secondary aggregation and release, provided that it has no clot-promoting activity.Abstract:
Extensive aggregation of human platelets can be induced by ADP without secondaryaggregation or release of granule contents. This occurs with washed platelets in Tyrode solution containing 0.35% alb...read more
Citations
More filters
Journal ArticleDOI
Human platelets possess an inducible and saturable receptor specific for fibrinogen
Journal ArticleDOI
ADP receptors of platelets and their inhibition.
TL;DR: Studies in P2Y1 knock-out mice and experimental thrombosis models using selective P2y1 antagonists have shown that the P2 Y1 receptor, like the P 2Y12 receptor, is a potential target for new antithrombotic drugs.
Journal ArticleDOI
Aspirin and clopidogrel: Efficacy, safety, and the issue of drug resistance
TL;DR: “Aspirin-resistant” should be considered as a description for those individuals in whom aspirin fails to inhibit thromboxane A2 production, irrespective of the results of unspecific tests of platelet function, such as the bleeding time, platelet aggregation, or the PFA-100 system.
Journal ArticleDOI
Interaction of fibrinogen with its platelet receptor as part of a multistep reaction in ADP-induced platelet aggregation.
TL;DR: The interaction of fibrinogen with platelets appears to involve multiple steps, and a model sequence for these reactions is proposed, and it is suggested that the continuous presence of ADP is required for the binding of fbrinogen.
Journal ArticleDOI
Interrelations of Platelet Aggregation and Secretion
TL;DR: The mechanism of stimulus-response coupling in human platelets was investigated with a new instrument that simultaneously monitors aggregation and secretion in the same sample of plateletrich plasma, finding little evidence for second wave aggregation being a consequence of secretion as usually described.
References
More filters
Journal ArticleDOI
Preparation of Suspensions of Washed Platelets from Humans
TL;DR: Methods have been developed for the preparation of suspensions of washed platelets from humans and platelets suspended in Eagle's tissue culture medium containing albumin were more responsive to ADP than platelets in Tyrode's‐albumin solution.
Journal ArticleDOI
Role of adenosine diphosphate in the aggregation of human blood-platelets by thrombin and by fatty acids.
Haslam Rj,Haslam Rj +1 more
Journal ArticleDOI
The release of nucleotides, 5‐hydroxytryptamine and enzymes from human blood platelets during aggregation
TL;DR: Adenosine diphosphate (ADP) and adrenaline caused the aggregation of human platelets suspended in plasma containing citrate anticoagulant and stirred at 37° C and the concentration of ADP appearing in the plasma was up to 7 times the concentration added.
Journal ArticleDOI
Factors affecting the adhesiveness of human leucocytes and platelets in vitro
TL;DR: There was a concurrent loss of the usual ability of the PMN to migrate, but at least in the presence of EDTA, a capacity to change shape by pseudopod formation remained and, within limits, this retention was not related to either flow rate or column length.