Journal ArticleDOI
Fate of teratocarcinoma cells injected into early mouse embryos
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It is shown here that embryonal carcinoma cells can participate in normal embryogenesis, thus providing further evidence for the validity of the use of these cultures as a model of normal embryonic development.Abstract:
ANALYSIS of early mammalian development is complicated by technical difficulties. The initial processes of cellular determination and differentiation in the mouse embryo take place in small populations of cells1,2, and major embryogenic events occur after uterine implantation when the embryo is largely inaccessible. Recent work, however, suggests that murine teratocarcinomas may provide a convenient model for studying mammalian development3–6. These are transplantable tumours of germ cell or embryonic cell origin3–6, typically consisting of a variety of differentiated tissues and undifferentiated stem cells. The stem cells, called embryonal carcinoma, resemble cells of early embryos in morphological, biochemical and cell surface properties, and in developmental potential3–6. They can be propagated in tissue culture to provide sufficient material for biochemical analysis. After inoculation into histocompatible adult hosts they form differentiated teratocarcinomas. They also differentiate in vitro7,8 where the first stages of their differentiation seem to parallel normal embryonic development. We show here that embryonal carcinoma cells can participate in normal embryogenesis, thus providing further evidence for the validity of the use of these cultures as a model of normal embryonic development.read more
Citations
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Journal ArticleDOI
Genetic transformation of mouse embryos by microinjection of purified DNA.
TL;DR: The results demonstrate that genes can be introduced into the mouse genome by direct insertion into the nuclei of early embryos by microinjected into pronuclei of fertilized mouse oocytes.
Journal ArticleDOI
Embryonic stem cells.
Helen J. Rippon,Anne E. Bishop +1 more
TL;DR: This work reviews the history of murine and human ES cell Lines, including practical and ethical aspects of ES cell isolation from pre‐implantation embryos, maintenance of undifferentiated ES cell lines in the cell culture environment, and differentiation of ES cells in vitro and in vivo into mature somatic cell types.
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Tumors as Organs: Complex Tissues that Interface with the Entire Organism
TL;DR: Understanding the complex ways in which cancer cells interact with their surroundings, both locally in the tumor organ and systemically in the body as a whole, has implications for effective cancer prevention and therapy.
Journal ArticleDOI
Embryonic Stem Cells: Prospects for Developmental Biology and Cell Therapy
Anna M. Wobus,Kenneth R. Boheler +1 more
TL;DR: This review focuses both on mouse and human ES cells with respect to in vitro propagation and differentiation as well as their use in basic cell and developmental biology and toxicology and presents prospects forhuman ES cells in tissue regeneration and transplantation.
Journal ArticleDOI
Teratocarcinomas and mammalian embryogenesis
TL;DR: The availability of stem cell lines isolated from mouse teratocarcinomas has made possible a number of new biochemical, immunological, and genetic approahes to the study of early mammalian development.
References
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Journal ArticleDOI
Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro.
Gail R. Martin,Martin J. Evans +1 more
TL;DR: There are similarities between the process of embryoid body formation and the early events of differentiation of the mouse embryo in vitro, and extensive differentiation to a wide variety of cell types occurs.
Journal ArticleDOI
The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos.
TL;DR: It is shown that a teratoma originates from a disorganized population of undifferentiated embryonic cells, and this population is derived from grafted embryos that resemble the spontaneous testicular teratomas characteristic of strain 129/Sv.
Journal ArticleDOI
The effect of cells transferred into the mouse blastocyst on subsequent development.
TL;DR: These experiments indicate that the transferred cells were able to establish small colonies in the embryos and that some of these cells persisted into the adult.
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