Journal ArticleDOI
Feedback phase correction of Bessel beams in confocal line light-sheet microscopy: a simulation study
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TLDR
This work presents a concept of how to correct the phase of each incident Bessel beam such that the efficiency of confocal line detection is improved by up to 200%-300%.Abstract:
Confocal line detection has been shown to improve contrast in light-sheet-based microscopy especially when illuminating the sample by Bessel beams. Besides their self-reconstructing capability, the stability in propagation direction of Bessel beams allows to block the unwanted emission light from the Bessel beam's ring system. However, due to phase aberrations induced especially at the border of the specimen, Bessel beams may not propagate along lines parallel to the slit detector. Here we present a concept of how to correct the phase of each incident Bessel beam such that the efficiency of confocal line detection is improved by up to 200%-300%. The applicability of the method is verified by the results obtained from numerical simulations based on the beam propagation method.read more
Citations
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Journal ArticleDOI
Adaptive optical microscopy: the ongoing quest for a perfect image
Martin J. Booth,Martin J. Booth +1 more
TL;DR: How technologies such as deformable mirrors and spatial light modulators, which compensate for aberrations by locally controlling the wavefront of a light wave, are now improving the performance of multiphoton, confocal, widefield and super-resolution microscopes are reviewed.
Journal ArticleDOI
The light-sheet microscopy revolution
TL;DR: In this article, the authors review the rapid advances that have been made in one form of optical biological imaging in the last decade, namely that of light-sheet microscopy, focusing on the crucial role that modern optical technology, frequently developed for other fields, has played in advancing the instrumentation.
Journal ArticleDOI
Fast imaging of live organisms with sculpted light sheets
Aleksander K. Chmielewski,Anders Kyrsting,Pierre Mahou,Matthew T. Wayland,Leila Muresan,Jan Felix Evers,Jan Felix Evers,Clemens F. Kaminski +7 more
TL;DR: This work presents a new, fast, flexible and simple to implement method to optimize the illumination light-sheet to the requirement at hand, and proves compatible with confocal line scanning detection, further improving image contrast and resolution.
Journal ArticleDOI
Separation of ballistic and diffusive fluorescence photons in confocal Light-Sheet Microscopy of Arabidopsis roots.
TL;DR: This work demonstrates that ballistic and diffusive fluorescence photons can be separated by analyzing the image spectra in each plane without a priori knowledge and introduces a theoretical model allowing to extract typical scattering parameters of the biological material.
Journal ArticleDOI
Characterizing the beam steering and distortion of Gaussian and Bessel beams focused in tissues with microscopic heterogeneities.
Ye Chen,Jonathan T. C. Liu +1 more
TL;DR: The results indicate that low-NA Bessel beams exhibit reduced beam-steering artifacts and distortions compared to Gaussian beams, and are therefore potentially useful for microscopy applications in which pointing accuracy and beam quality are critical, such as dual-axis confocal (DAC) microscopy.
References
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Journal ArticleDOI
Optical sectioning deep inside live embryos by selective plane illumination microscopy
TL;DR: In this article, a selective plane illumination microscopy (SPIM) was developed to generate multidimensional images of samples up to a few millimeters in size, which can be applied to visualize the embryogenesis of the relatively opaque Drosophila melanogaster in vivo.
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Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy.
TL;DR: This work developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development to derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event.
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Light propagation in graded-index optical fibers
Michael D. Feit,J. A. Fleck +1 more
TL;DR: An accurate numerical method is described for solving the Helmholtz equation for a general class of optical fibers and yields detailed information about the spatial and angular properties of the propagating beam as well as the modal propagation constants for the fiber.
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Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
Thomas A. Planchon,Liang Gao,Daniel E. Milkie,Michael W. Davidson,James A. Galbraith,Catherine G. Galbraith,Eric Betzig +6 more
TL;DR: Scanned Bessel beams are used in conjunction with structured illumination and/or two-photon excitation to create thinner light sheets better suited to three-dimensional (3D) subcellular imaging.
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Principles and techniques of digital holographic microscopy
TL;DR: Digital holography is an emerging field of new paradigm in general imaging applications as discussed by the authors, and a review of a subset of the research and development activities in digital holographic microscopy techniques and applications is presented.