Journal ArticleDOI
Femtomolar Sensitivity of Metalloregulatory Proteins Controlling Zinc Homeostasis
Reads0
Chats0
TLDR
The mechanism of zinc sensors that control metal uptake or export in Escherichia coli are determined and their response against the thermodynamically defined free zinc concentration suggests an extraordinary intracellular zinc-binding capacity.Abstract:
Intracellular zinc is thought to be available in a cytosolic pool of free or loosely bound Zn(II) ions in the micromolar to picomolar range To test this, we determined the mechanism of zinc sensors that control metal uptake or export in Escherichia coli and calibrated their response against the thermodynamically defined free zinc concentration Whereas the cellular zinc quota is millimolar, free Zn(II) concentrations that trigger transcription of zinc uptake or efflux machinery are femtomolar, or six orders of magnitude less than one atom per cell This is not consistent with a cytosolic pool of free Zn(II) and suggests an extraordinary intracellular zinc-binding capacity Thus, cells exert tight control over cytosolic metal concentrations, even for relatively low-toxicity metals such as zincread more
Citations
More filters
Journal ArticleDOI
A study on the dynamics of the zraP gene expression profile and its application to the construction of zinc adsorption bacteria
TL;DR: Results indicate that the engineered bacterial strain developed in the present study can sense the specific heavy metal and activates a cell surface display system that acts to remove the metal.
Journal ArticleDOI
Water molecules mediate zinc mobility in the bacterial zinc diffusion channel ZIPB.
TL;DR: The unprecedented details of water-mediated zinc transport identified here highlight an essential role of solvated waters in driving zinc coordination dynamics and transmembrane crossing.
Journal ArticleDOI
How cyanophage S-2L rejects adenine and incorporates 2-aminoadenine to saturate hydrogen bonding in its DNA
Dariusz Czernecki,Pierre Legrand,Pierre Legrand,Mustafa Tekpinar,Sandrine Rosario,Pierre-Alexandre Kaminski,Marc Delarue +6 more
TL;DR: In this paper, a member of the PrimPol family is identified as the sole possible polymerase of S-2L and it can incorporate both A and Z in front of a T.
Journal ArticleDOI
The Components of the Unique Zur Regulon of Cupriavidus metallidurans Mediate Cytoplasmic Zinc Handling.
TL;DR: A compartmentalization of zinc homeostasis in C. metallidurans is shown, where the periplasm is responsible for the removal of surplus zinc, cytoplasmic components areresponsible for the management of zinc as an essential cofactor, and the two compartments are connected by ZupT.
Journal ArticleDOI
Cobalt Resistance via Detoxification and Mineralization in the Iron-Reducing Bacterium Geobacter sulfurreducens.
TL;DR: Transcriptomic evidence revealed pathways for cell envelope modification that increased metal resistance and promoted cell-cell aggregation and biofilm formation in stationary phase that confer on Geobacter a competitive advantage for growth in metal-rich environments that are essential to the sustainability of cobamide-dependent microbiomes.
References
More filters
Journal ArticleDOI
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
Journal ArticleDOI
The Galvanization of Biology: A Growing Appreciation for the Roles of Zinc
Jeremy M Berg,Yigong Shi +1 more
TL;DR: The ability of zinc to be bound specifically within a range of tetrahedral sites appears to be responsible for the evolution of the wide range of zinc-stabilized structural domains now known to exist.
Journal ArticleDOI
Undetectable intracellular free copper: the requirement of a copper chaperone for superoxide dismutase.
TL;DR: Results indicate that intracellular [Cu]free is limited to less than one free copper ion per cell and suggest that a pool of free copper ions is not used in physiological activation of metalloenzymes.
Book
Physiology of the bacterial cell : a molecular approach
TL;DR: Composition and organization of the bacterial cell structure and function of bacterial cell parts assembly and polymerization, multigene systems and global regulation cell cycle growth rate as a variable cellular differentiation physiological ecology answers to study questions literature cited.