Journal ArticleDOI
Femtomolar Sensitivity of Metalloregulatory Proteins Controlling Zinc Homeostasis
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TLDR
The mechanism of zinc sensors that control metal uptake or export in Escherichia coli are determined and their response against the thermodynamically defined free zinc concentration suggests an extraordinary intracellular zinc-binding capacity.Abstract:
Intracellular zinc is thought to be available in a cytosolic pool of free or loosely bound Zn(II) ions in the micromolar to picomolar range To test this, we determined the mechanism of zinc sensors that control metal uptake or export in Escherichia coli and calibrated their response against the thermodynamically defined free zinc concentration Whereas the cellular zinc quota is millimolar, free Zn(II) concentrations that trigger transcription of zinc uptake or efflux machinery are femtomolar, or six orders of magnitude less than one atom per cell This is not consistent with a cytosolic pool of free Zn(II) and suggests an extraordinary intracellular zinc-binding capacity Thus, cells exert tight control over cytosolic metal concentrations, even for relatively low-toxicity metals such as zincread more
Citations
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Phytoremediation experiments with Helianthus tuberosus under different pH and heavy metal soil concentrations
TL;DR: In this paper, experiments for the investigation of phytoextraction and growth behavior of Helianthus tuberosus under different soil pH values and heavy metal concentrations were performed.
Journal ArticleDOI
Development of a Pigment-Based Whole-Cell Zinc Biosensor for Human Serum
TL;DR: This work presents a proof of principle for the development of low-cost, minimal-equipment, field-deployable biosensors for nutritional epidemiology applications, and presents approaches to overcome innate immune responses that would otherwise hinder bacterial sensor survival.
Journal ArticleDOI
How to hide zinc in a small protein
TL;DR: Genome sequences suggest that related zinc-finger sites are common in a variety of bacteria and can exert fine control over both the thermodynamics and kinetics of zinc binding in thiolate clusters.
Journal ArticleDOI
The role of the cytoplasmic heme-binding protein (PhuS) of Pseudomonas aeruginosa in intracellular heme trafficking and iron homeostasis.
TL;DR: The present data suggest that PhuS has a dual function in trafficking heme to HO, and in directly or indirectly sensing and maintaining iron and heme homeostasis.
Journal ArticleDOI
Ligand Binding Induces an Ammonia Channel in 2-Amino-2-desoxyisochorismate (ADIC) Synthase PhzE
Qi Ang Li,Dmitri V. Mavrodi,Linda S. Thomashow,Linda S. Thomashow,Manfred Roessle,Wulf Blankenfeldt +5 more
TL;DR: The assignment of PhzE as an ADIC synthase was confirmed by mass spectrometric analysis of the product and additional electron density in the GATase1 active center was identified as zinc, and it was demonstrated that Zn2+, Mn2+, and Ni2+ reduce the activity of PhZE.
References
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One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
Journal ArticleDOI
The Galvanization of Biology: A Growing Appreciation for the Roles of Zinc
Jeremy M Berg,Yigong Shi +1 more
TL;DR: The ability of zinc to be bound specifically within a range of tetrahedral sites appears to be responsible for the evolution of the wide range of zinc-stabilized structural domains now known to exist.
Journal ArticleDOI
Undetectable intracellular free copper: the requirement of a copper chaperone for superoxide dismutase.
TL;DR: Results indicate that intracellular [Cu]free is limited to less than one free copper ion per cell and suggest that a pool of free copper ions is not used in physiological activation of metalloenzymes.
Book
Physiology of the bacterial cell : a molecular approach
TL;DR: Composition and organization of the bacterial cell structure and function of bacterial cell parts assembly and polymerization, multigene systems and global regulation cell cycle growth rate as a variable cellular differentiation physiological ecology answers to study questions literature cited.