Journal ArticleDOI
Femtomolar Sensitivity of Metalloregulatory Proteins Controlling Zinc Homeostasis
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TLDR
The mechanism of zinc sensors that control metal uptake or export in Escherichia coli are determined and their response against the thermodynamically defined free zinc concentration suggests an extraordinary intracellular zinc-binding capacity.Abstract:
Intracellular zinc is thought to be available in a cytosolic pool of free or loosely bound Zn(II) ions in the micromolar to picomolar range To test this, we determined the mechanism of zinc sensors that control metal uptake or export in Escherichia coli and calibrated their response against the thermodynamically defined free zinc concentration Whereas the cellular zinc quota is millimolar, free Zn(II) concentrations that trigger transcription of zinc uptake or efflux machinery are femtomolar, or six orders of magnitude less than one atom per cell This is not consistent with a cytosolic pool of free Zn(II) and suggests an extraordinary intracellular zinc-binding capacity Thus, cells exert tight control over cytosolic metal concentrations, even for relatively low-toxicity metals such as zincread more
Citations
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Molecular Mechanism of ISC Iron–Sulfur Cluster Biogenesis Revealed by High-Resolution Native Mass Spectrometry
TL;DR: Results support an iron-first model for Fe-S cluster synthesis and highlight the power of native MS in defining protein-associated intermediates and elucidating mechanistic details of enzymatic processes.
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Intracellular Pb2+ Content Monitoring Using a Protein-Based Pb2+ Indicator
TL;DR: A new Pb(2+) indicator, Met-lead 1.59, was successfully developed for advanced research on Pb (2+) toxicology and successfully monitored the intracellular Pb-2+) content in human embryonic kidney cells under both short- and long-term treatments.
Journal ArticleDOI
Zinc transporter 8 (ZnT8) expression is reduced by ischemic insults: a potential therapeutic target to prevent ischemic retinopathy.
Michael DeNiro,Futwan Al-Mohanna +1 more
TL;DR: The data indicate that ischemic retinopathy maybe mediated by aberrant Zn++ homeostasis caused by ZnT8 downregulation, whereas YC-1 plays a neuroprotective role against isChemic insult, which provides a therapeutic strategy to combat neovascular eye diseases.
Journal ArticleDOI
Characterization of Escherichia coli D-arabinose 5-phosphate isomerase encoded by kpsF: implications for group 2 capsule biosynthesis.
TL;DR: K-API is now the second Kdo biosynthetic related gene that has been characterized from the kps group 2 capsule cluster, and most of the properties are quite similar to its LPS API counterpart KdsD, but the catalytic constant is nearly 10-fold lower.
Journal ArticleDOI
Calculating metalation in cells reveals CobW acquires CoII for vitamin B12 biosynthesis while related proteins prefer ZnII
Tessa R. Young,Maria Alessandra Martini,Maria Alessandra Martini,Andrew W. Foster,Arthur Glasfeld,Deenah Osman,Richard Morton,Evelyne Deery,Martin J. Warren,Martin J. Warren,Nigel J. Robinson +10 more
TL;DR: In this paper, the available free energies of metals have been determined from the responses of metal sensors, and the free energy values were used to develop a metalation-calculator which accounts for inter-metal competition and changing metal-availabilities inside cells.
References
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Journal ArticleDOI
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
Journal ArticleDOI
The Galvanization of Biology: A Growing Appreciation for the Roles of Zinc
Jeremy M Berg,Yigong Shi +1 more
TL;DR: The ability of zinc to be bound specifically within a range of tetrahedral sites appears to be responsible for the evolution of the wide range of zinc-stabilized structural domains now known to exist.
Journal ArticleDOI
Undetectable intracellular free copper: the requirement of a copper chaperone for superoxide dismutase.
TL;DR: Results indicate that intracellular [Cu]free is limited to less than one free copper ion per cell and suggest that a pool of free copper ions is not used in physiological activation of metalloenzymes.
Book
Physiology of the bacterial cell : a molecular approach
TL;DR: Composition and organization of the bacterial cell structure and function of bacterial cell parts assembly and polymerization, multigene systems and global regulation cell cycle growth rate as a variable cellular differentiation physiological ecology answers to study questions literature cited.