Journal ArticleDOI
Femtomolar Sensitivity of Metalloregulatory Proteins Controlling Zinc Homeostasis
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TLDR
The mechanism of zinc sensors that control metal uptake or export in Escherichia coli are determined and their response against the thermodynamically defined free zinc concentration suggests an extraordinary intracellular zinc-binding capacity.Abstract:
Intracellular zinc is thought to be available in a cytosolic pool of free or loosely bound Zn(II) ions in the micromolar to picomolar range To test this, we determined the mechanism of zinc sensors that control metal uptake or export in Escherichia coli and calibrated their response against the thermodynamically defined free zinc concentration Whereas the cellular zinc quota is millimolar, free Zn(II) concentrations that trigger transcription of zinc uptake or efflux machinery are femtomolar, or six orders of magnitude less than one atom per cell This is not consistent with a cytosolic pool of free Zn(II) and suggests an extraordinary intracellular zinc-binding capacity Thus, cells exert tight control over cytosolic metal concentrations, even for relatively low-toxicity metals such as zincread more
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Comparative study of the active cadmium efflux systems operating at the plasma membrane and tonoplast of cucumber root cells
TL;DR: The overall calculation of Cd accumulation in the everted plasma membranes and vacuolar vesicles suggests that the tonoplast and vacUole have a major function in Cd efflux from the cytosol in the roots of cucumber subjected to Cd stress.
Journal ArticleDOI
Response to kainic acid injections: changes in staining for zinc, FOS, cell death and glial response in the rat forebrain.
À. Riba-Bosch,J. Pérez-Clausell +1 more
TL;DR: Cytoplasmic zinc (zinquin) was coincident, in time and location, with cell degeneration, thus implicating zinc in cell death, and various pools of zinc after kainate injection were revealed.
Journal ArticleDOI
Functional characterization of iron-substituted tristetraprolin-2D (TTP-2D, NUP475-2D): RNA binding affinity and selectivity.
TL;DR: The iron-binding properties of TTP-2D and the effect of iron substitution on RNA recognition have been investigated and suggest that iron is a viable substitute for zinc in this protein.
Journal ArticleDOI
Terminal oxidation and the effects of zinc in prostate versus liver mitochondria
TL;DR: ZnLigands were equally as effective as free Zn++ ions in the inhibition of respiration and terminal oxidation of both prostate and liver mitochondria, which supports the concept that zinc can be transferred from cytosolic donor Zn Ligands directly to zinc-binding sites of terminal oxidation components.
Journal ArticleDOI
The requirement for cobalt in vitamin B12: A paradigm for protein metalation.
Deenah Osman,Anastasia Cooke,Tessa R. Young,Evelyne Deery,Nigel J. Robinson,Martin J. Warren +5 more
TL;DR: The partitioning of cobalt for cobalamin biosynthesis exemplifies how cells assist metalation and is investigated to investigate why cobalt is paired specifically with the corrin ring, how Cobalt is inserted during the biosynthetic process, how cobALT is made available within the cell and explore the cellular control of cobALT and cobalam levels.
References
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Journal ArticleDOI
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
Journal ArticleDOI
The Galvanization of Biology: A Growing Appreciation for the Roles of Zinc
Jeremy M Berg,Yigong Shi +1 more
TL;DR: The ability of zinc to be bound specifically within a range of tetrahedral sites appears to be responsible for the evolution of the wide range of zinc-stabilized structural domains now known to exist.
Journal ArticleDOI
Undetectable intracellular free copper: the requirement of a copper chaperone for superoxide dismutase.
TL;DR: Results indicate that intracellular [Cu]free is limited to less than one free copper ion per cell and suggest that a pool of free copper ions is not used in physiological activation of metalloenzymes.
Book
Physiology of the bacterial cell : a molecular approach
TL;DR: Composition and organization of the bacterial cell structure and function of bacterial cell parts assembly and polymerization, multigene systems and global regulation cell cycle growth rate as a variable cellular differentiation physiological ecology answers to study questions literature cited.