Journal ArticleDOI
Femtomolar Sensitivity of Metalloregulatory Proteins Controlling Zinc Homeostasis
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TLDR
The mechanism of zinc sensors that control metal uptake or export in Escherichia coli are determined and their response against the thermodynamically defined free zinc concentration suggests an extraordinary intracellular zinc-binding capacity.Abstract:
Intracellular zinc is thought to be available in a cytosolic pool of free or loosely bound Zn(II) ions in the micromolar to picomolar range To test this, we determined the mechanism of zinc sensors that control metal uptake or export in Escherichia coli and calibrated their response against the thermodynamically defined free zinc concentration Whereas the cellular zinc quota is millimolar, free Zn(II) concentrations that trigger transcription of zinc uptake or efflux machinery are femtomolar, or six orders of magnitude less than one atom per cell This is not consistent with a cytosolic pool of free Zn(II) and suggests an extraordinary intracellular zinc-binding capacity Thus, cells exert tight control over cytosolic metal concentrations, even for relatively low-toxicity metals such as zincread more
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Journal ArticleDOI
Bacterial Production of Pinene by a Laboratory-Evolved Pinene-Synthase
Miki Tashiro,Hiroshi Kiyota,Shigeko Kawai-Noma,Kyoichi Saito,Masahiko Ikeuchi,Yoko Iijima,Daisuke Umeno,Daisuke Umeno +7 more
TL;DR: A pinene synthase variant was isolated that outperformed the wild-type (parent) enzyme in multiple contexts in Escherichia coli and cyanobacteria and exhibited drastically altered metal dependency, enabling to keep the activity in the cytosol that is manganese-deficient.
Journal ArticleDOI
Quercetinase QueD of Streptomyces sp. FLA, a Monocupin Dioxygenase with a Preference for Nickel and Cobalt†
TL;DR: On the basis of spectroscopic data, and considering the organic chemistry of flavonols, the nonredox role of the metal center in catalysis is discussed.
Journal ArticleDOI
Role of Zinc Nutrition for Increasing Zinc Availability, Uptake, Yield, and Quality of Maize (Zea Mays L.) Grains: An Overview
TL;DR: The magnitude of zinc deficiency varies widely among soil types with reduction in crop yield and quality to the tune of 25–35% and questions the nutritional security of human beings in developing countries like India, where cereals are the staple food.
Journal ArticleDOI
The Metal Homeostasis Protein, Lsp, of Streptococcus pyogenes Is Necessary for Acquisition of Zinc and Virulence
TL;DR: It is suggested that Lsp is a ligand-binding component of an ABC-type zinc permease and that perturbation of zinc homeostasis inhibits the ability of S. pyogenes to cause disease in a zinc-limited host milieu.
Book ChapterDOI
Catalytic mechanisms of metallohydrolases containing two metal ions.
Nataša Mitić,Manfredi Miraula,Christopher Selleck,Kieran S. Hadler,Elena Uribe,Marcelo Monteiro Pedroso,Gerhard Schenk +6 more
TL;DR: This chapter discusses and compares the reaction mechanisms of several closely related enzymes with a view to highlighting the functional diversity bestowed upon them by their metal ion cofactors.
References
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Journal ArticleDOI
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
Journal ArticleDOI
The Galvanization of Biology: A Growing Appreciation for the Roles of Zinc
Jeremy M Berg,Yigong Shi +1 more
TL;DR: The ability of zinc to be bound specifically within a range of tetrahedral sites appears to be responsible for the evolution of the wide range of zinc-stabilized structural domains now known to exist.
Journal ArticleDOI
Undetectable intracellular free copper: the requirement of a copper chaperone for superoxide dismutase.
TL;DR: Results indicate that intracellular [Cu]free is limited to less than one free copper ion per cell and suggest that a pool of free copper ions is not used in physiological activation of metalloenzymes.
Book
Physiology of the bacterial cell : a molecular approach
TL;DR: Composition and organization of the bacterial cell structure and function of bacterial cell parts assembly and polymerization, multigene systems and global regulation cell cycle growth rate as a variable cellular differentiation physiological ecology answers to study questions literature cited.