Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo.
Sunjay Kaushal,Gilad E. Amiel,Kristine J. Guleserian,Oz M. Shapira,Tjorvi E. Perry,Fraser W.H Sutherland,Elena Rabkin,Adrian M. Moran,Frederick J. Schoen,Anthony Atala,Shay Soker,Joyce Bischoff,John E. Mayer +12 more
TLDR
Results indicate that EPCs can function similarly to arterial endothelial cells and thereby confer longer vascular-graft survival and might have other general applications for tissue-engineered structures and in treating vascular diseases.Abstract:
Arterial conduits are increasingly preferred for surgical bypass because of inherent functional properties conferred by arterial endothelial cells, especially nitric oxide production in response to physiologic stimuli Here we tested whether endothelial progenitor cells (EPCs) can replace arterial endothelial cells and promote patency in tissue-engineered small-diameter blood vessels (4 mm) We isolated EPCs from peripheral blood of sheep, expanded them ex vivo and then seeded them on decellularized porcine iliac vessels EPC-seeded grafts remained patent for 130 days as a carotid interposition graft in sheep, whereas non-seeded grafts occluded within 15 days The EPC-explanted grafts exhibited contractile activity and nitric-oxide-mediated vascular relaxation that were similar to native carotid arteries These results indicate that EPCs can function similarly to arterial endothelial cells and thereby confer longer vascular-graft survival Due to their unique properties, EPCs might have other general applications for tissue-engineered structures and in treating vascular diseasesread more
Citations
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Circulating Endothelial Progenitor Cells, Vascular Function, and Cardiovascular Risk
Jonathan Hill,Gloria Zalos,Julian Halcox,William H. Schenke,Myron A. Waclawiw,Arshed A. Quyyumi,Toren Finkel +6 more
TL;DR: A strong correlation between the number of circulating endothelial progenitor cells and the subjects' combined Framingham risk factor score was observed and measurement of flow-mediated brachial-artery reactivity revealed a signifi...
Journal ArticleDOI
Endothelial Progenitor Cells: Characterization and Role in Vascular Biology
Carmen Urbich,Stefanie Dimmeler +1 more
TL;DR: This review summarizes the mechanisms regulating endothelial progenitor cell–mediated neovascularization and reendothelialization and describes the characterization of the different progenitors cell populations.
Journal ArticleDOI
Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration.
Shahin Rafii,David Lyden +1 more
TL;DR: Identification of factors that promote differentiation of the progenitor cells will permit functional incorporation into neo-vessels of specific tissues while diminishing potential toxicity to other organs.
Journal ArticleDOI
Hematopoietic stem cells differentiate into vascular cells that participate in the pathogenesis of atherosclerosis
Masataka Sata,Akio Saiura,Atsushi Kunisato,Akihiro Tojo,Seiji Okada,Takeshi Tokuhisa,Hisamaru Hirai,Masatoshi Makuuchi,Yasunobu Hirata,Ryozo Nagai +9 more
TL;DR: It is shown that in models of post-angioplasty restenosis, graft vasculopathy and hyperlipidemia-induced atherosclerosis, bone-marrow cells give rise to most of the SMCs that contribute to arterial remodeling, indicating that somatic stem cells contribute to pathological remodeling of remote organs.
References
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Isolation of putative progenitor endothelial cells for angiogenesis.
Takayuki Asahara,Toyoaki Murohara,Alison Sullivan,Marcy Silver,Rien van der Zee,Tong Li,Bernhard Witzenbichler,Gina C. Schatteman,Jeffrey M. Isner +8 more
TL;DR: It is suggested that EC progenitors may be useful for augmenting collateral vessel growth to ischemic tissues (therapeutic angiogenesis) and for delivering anti- or pro-angiogenic agents, respectively, to sites of pathologic or utilitarianAngiogenesis.
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Bone Marrow Origin of Endothelial Progenitor Cells Responsible for Postnatal Vasculogenesis in Physiological and Pathological Neovascularization
Takayuki Asahara,Haruchika Masuda,Tomono Takahashi,Christoph Kalka,Christopher Pastore,Marcy Silver,Marianne Kearne,Meredith Magner,Jeffrey M. Isner +8 more
TL;DR: Findings indicate that postnatal neovascularization does not rely exclusively on sprouting from preexisting blood vessels (angiogenesis); instead, EPCs circulate from bone marrow to incorporate into and thus contribute to postnatal physiological and pathological neov vascularization, which is consistent with postnatal vasculogenesis.
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Ischemia- and cytokine-induced mobilization of bone marrow-derived endothelial progenitor cells for neovascularization.
Tomono Takahashi,Christoph Kalka,Haruchika Masuda,Donghui Chen,Marcy Silver,Marianne Kearney,Meredith Magner,Jeffrey M. Isner,Takayuki Asahara +8 more
TL;DR: Findings indicate that circulating EPCs are mobilized endogenously in response to tissue ischemia or exogenously by cytokine therapy and thereby augment neovascularization of ischemic tissues.
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Expression of VEGFR-2 and AC133 by circulating human CD34+ cells identifies a population of functional endothelial precursors
Mario Peichev,Afzal J. Naiyer,Daniel S. Pereira,Zhenping Zhu,William J. Lane,Mathew R. Williams,Mehmet C. Oz,Daniel J. Hicklin,Larry Witte,Malcolm A.S. Moore,Shahin Rafii +10 more
TL;DR: In an in vivo human model, it is found that the neo-intima formed on the surface of left ventricular assist devices is colonized with AC133(+)VEGFR-2(+) cells, suggesting a phenotypically and functionally distinct population of circulating endothelial cells that may play a role in neo-angiogenesis.
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Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization.
Christoph Kalka,Haruchika Masuda,Tomono Takahashi,Wiltrud M. Kalka-Moll,Marcy Silver,Marianne Kearney,Tong Li,Jeffrey M. Isner,Takayuki Asahara +8 more
TL;DR: Ex vivo expanded hEPCs may have utility as a "supply-side" strategy for therapeutic neovascularization in mice with hindlimb ischemia and the rate of limb loss was significantly reduced.