Journal ArticleDOI
Genome-wide identification and characterization of maize expansin genes expressed in endosperm.
Wei Zhang,Hanwei Yan,Weijun Chen,Jinyang Liu,Cuiping Jiang,Haiyang Jiang,Suwen Zhu,Beijiu Cheng +7 more
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TLDR
A systematic analysis of the expansin gene family encompassing gene structure, phylogeny, chromosomal location, gene duplication, and gene ontology provides essential information for ZmEXPs cloning and functional exploration and will assist research on expansin-related mechanisms and contribute to future enhancement of maize grain yield.Abstract:
By promoting cell wall loosening, expansins contribute to cell enlargement during various developmental processes. Nevertheless, the role of expansins in the expansion and development of endosperm—a major seed component whose cell size is significantly associated with grain yield—is poorly understood. To explore associated biological processes and the evolution of expansins in maize, we performed a systematic analysis of the expansin gene family encompassing gene structure, phylogeny, chromosomal location, gene duplication, and gene ontology. A total of 88 maize expansin genes (ZmEXPs) were identified and categorized into three subfamilies according to their phylogenetic relationships. Expression patterns of ZmEXPs were also investigated in nine different tissues by semi-quantitative RT-PCR. The expression of eight ZmEXPs was detected in endosperm, with five showing endosperm-specific expression. Quantitative RT-PCR was used to analyze expression patterns of the eight ZmEXPs in endosperm (10 days after pollination) under abscisic acid (ABA) and gibberellic acid (GA3) treatments. All eight ZmEXPs were found to be significantly regulated by ABA and GA3 in endosperm, suggesting important roles for these hormones in the regulation of ZmEXPs during endosperm development. Our results provide essential information for ZmEXPs cloning and functional exploration, which will assist research on expansin-related mechanisms and contribute to future enhancement of maize grain yield.read more
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Journal ArticleDOI
Plant expansins: diversity and interactions with plant cell walls
TL;DR: Molecular analyses of expansins from bacteria have identified residues essential for wall loosening activity and clarified the bifunctional nature of expansin binding to complex cell walls.
Journal ArticleDOI
Expansins: roles in plant growth and potential applications in crop improvement
TL;DR: Some of the reported functions of expansins are reviewed and summarized and the potential uses of expansin in crop improvement programs are outlined.
Journal ArticleDOI
Drought stress delays endosperm development and misregulates genes associated with cytoskeleton organization and grain quality proteins in developing wheat seeds
Kevin Begcy,Harkamal Walia +1 more
TL;DR: The results provide new insights on how some of the early seed developmental events are impacted by water stress, and the underlying molecular pathways that can possibly impact both grain size and quality in wheat.
Journal ArticleDOI
Genome-wide identification of the expansin gene family in tobacco (Nicotiana tabacum).
TL;DR: This work provides solid background information related to structure, evolution and expression as well as regulatory cis-acting elements of the tobacco expansins that will provide a strong foundation for cloning and functional exploration of expansin genes in tobacco.
Journal ArticleDOI
Genome-wide identification and expression analysis of the expansin gene family in tomato.
TL;DR: Bioinformatic analysis showed that although tomato expansins share similarities with those from other plants, they also exhibit specific features regarding genetic structure and amino acid sequences, which indicates a unique evolutionary process.
References
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Journal ArticleDOI
Analysis of relative gene expression data using real-time quantitative pcr and the 2(-delta delta c(t)) method
TL;DR: The 2-Delta Delta C(T) method as mentioned in this paper was proposed to analyze the relative changes in gene expression from real-time quantitative PCR experiments, and it has been shown to be useful in the analysis of realtime, quantitative PCR data.
Journal ArticleDOI
Clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
TL;DR: The sensitivity of the commonly used progressive multiple sequence alignment method has been greatly improved and modifications are incorporated into a new program, CLUSTAL W, which is freely available.
Journal ArticleDOI
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
TL;DR: Version 4 of MEGA software expands on the existing facilities for editing DNA sequence data from autosequencers, mining Web-databases, performing automatic and manual sequence alignment, analyzing sequence alignments to estimate evolutionary distances, inferring phylogenetic trees, and testing evolutionary hypotheses.
Journal ArticleDOI
DnaSP v5
Pablo Librado,Julio Rozas +1 more
TL;DR: Version 5 implements a number of new features and analytical methods allowing extensive DNA polymorphism analyses on large datasets, including visualizing sliding window results integrated with available genome annotations in the UCSC browser.
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SignalP 4.0: discriminating signal peptides from transmembrane regions
Thomas Nordahl Petersen,Søren Brunak,Søren Brunak,Gunnar von Heijne,Gunnar von Heijne,Henrik Nielsen +5 more
TL;DR: SignalP 4.0 was the best signal-peptide predictor for all three organism types but was not in all cases as good as SignalP 3.0 according to cleavage-site sensitivity or signal- peptide correlation when there are no transmembrane proteins present.