Journal ArticleDOI
Glial cells in the pineal gland of mice and rats. A combined immunofluorescence and electron-microscopic study.
TLDR
In this paper, the pineal gland of mouse and rat using double immunolabeling techniques was investigated for the detection of antigenic markers characteristic of astrocytes and their differentiative states (i.e., glial fibrillary acidic protein (GFAP), vimentin, and M1 and C1 antigens).Abstract:
Antigenic markers characteristic of astrocytes and their differentiative states (i.e., glial fibrillary acidic protein (GFAP), vimentin, and M1 and C1 antigens) were investigated in the pineal gland of mouse and rat using double immunolabeling techniques. In both species the socalled interstitial cells as characterized by TEM were shown to be astrocytes, since they expressed vimentin, but neither fibronectin (a marker for fibroblasts and endothelial cells) nor the neuron-specific L1 antigen or tetanus toxin receptors. Subpopulations of vimentin-positive pineal astrocytes were also GFAP- and C1- antigen-positive. M1- antigenpositive cells were not detected.read more
Citations
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Differentiation-associated Na+-dependent inorganic phosphate cotransporter (DNPI) is a vesicular glutamate transporter in endocrine glutamatergic systems.
Mitsuko Hayashi,Masato Otsuka,Riyo Morimoto,Sumiko Hirota,Shouki Yatsushiro,Jun Takeda,Akitsugu Yamamoto,Yoshinori Moriyama +7 more
TL;DR: DNPI is preferentially expressed in mammalian pinealocytes, αTC6 cells, clonal pancreatic α cells, and α cells of Langerhans islets, which constitute evidence that DNPI functions as another vesicular transporter in glutamatergic endocrine cells as well as in neurons.
Journal ArticleDOI
Immunohistochemical localization of D-aspartate in the rat pineal gland.
Jen Ai Lee,Hiroshi Homma,Kumiko Sakai,Takeshi Fukushima,Tomofumi Santa,Ken Tashiro,Takeshi Iwatsubo,Masayoshi Yoshikawa,Kazuhiro Imai +8 more
TL;DR: Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland, and this may indicate some yet unknown role in the regulation of melatonin secretion.
Journal ArticleDOI
Immunocytochemical and electron-microscopic characterization of macrophage/microglia cells and expression of class II major histocompatibility complex in the pineal gland of the rat
TL;DR: Cells expressing MHC class II antigen had a distribution and morphology similar to OX-42-immunopositive cells, suggesting that pineal macrophages/microglia play a role as antigen-presenting cells in the circumventricular neuroendocrine gland.
Journal ArticleDOI
Presence of glial cells in the rat pineal gland: A light and electron microscopic immunohistochemical study
TL;DR: Light and electron microscopic results showed the presence of these antigenic glial markers in the second pineal cell type and the term glial cell is proposed for the second of parenchymatous cell in rat pineal gland.
Journal ArticleDOI
Co-expression of vesicular glutamate transporters (VGLUT1 and VGLUT2) and their association with synaptic-like microvesicles in rat pinealocytes
Riyo Morimoto,Mitsuko Hayashi,Shouki Yatsushiro,Masato Otsuka,Akitsugu Yamamoto,Yoshinori Moriyama +5 more
TL;DR: It is shown that pinealocytes also express VGLUT1, and immunoelectronmicroscopy as well as subcellular fractionation studies revealed that both V GLUT1 and VGLut2 are specifically associated with SLMVs.
References
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Journal ArticleDOI
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TL;DR: It is not yet certain what pbysiobgical~dependontbepineal clock for cues is, but there is evidence at band ~ggest that the pineal participates in sanewayintbequlationoftbegonads,orsexglands.
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Immunocytological and biochemical characterization of a new neuronal cell surface component (L1 antigen) which is involved in cell adhesion.
TL;DR: During cerebellar development L1 antigen is detectable on tetanus toxin‐positive cells as early as embryonic day 13 after 3 days in culture and in the adult cerebellum, where it is predominantly localized in the molecular layer and around Purkinje cells.
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Immunocytochemical demonstration of vimentin in astrocytes and ependymal cells of developing and adult mouse nervous system.
TL;DR: The results show that vimentin and GFA protein coexist in one cell type not only in primary cultures in vitro but also in the intact tissue in situ.
Journal ArticleDOI
Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates.
TL;DR: The data are consistent with the idea that most cells grown in culture contain intermediate-sized filaments of the vimentin type, irrespective of the state of differentiation of the cells from which they are derived, and suggest that the vimentsin molecule contains both sequences conserved during evolution and regions different in different vertebrate species.