Globular and asymmetric acetylcholinesterase in frog muscle basal lamina sheaths.
TLDR
Observations show that all acetylcholinesterase forms can be accumulated in frog muscle BL and in the motor endplate-rich region of control muscle.Abstract:
After denervation in vivo, the frog cutaneus pectoris muscle can be led to degenerate by sectioning the muscle fibers on both sides of the region rich in motor endplate, leaving, 2 wk later, a muscle bridge containing the basal lamina (BL) sheaths of the muscle fibers (28). This preparation still contains various tissue remnants and some acetylcholine receptor-containing membranes. A further mild extraction by Triton X-100, a nonionic detergent, gives a pure BL sheath preparation, devoid of acetylcholine receptors. At the electron microscope level, this latter preparation is essentially composed of the muscle BL with no attached plasmic membrane and cellular component originating from Schwann cells or macrophages. Acetylcholinesterase is still present in high amounts in this BL sheath preparation. In both preparations, five major molecular forms (18, 14, 11, 6, and 3.5 S) can be identified that have either an asymmetric or a globular character. Their relative amount is found to be very similar in the BL and in the motor endplate-rich region of control muscle. Thus, observations show that all acetylcholinesterase forms can be accumulated in frog muscle BL.read more
Citations
More filters
Book ChapterDOI
Vertebrate Cholinesterases: Structure and Types of Interaction
J. Massoulié,J.-P. Toutant +1 more
TL;DR: It is found useful first to classify them as molecular forms, according to their hydrodynamic parameters (sedimentation coefficient, Stokes radius), corresponding to different quaternary structures.
Journal ArticleDOI
Distribution and role in regeneration of N-CAM in the basal laminae of muscle and Schwann cells.
François Rieger,Marc Nicolet,Martine Pinçon-Raymond,Monique Murawsky,Giovanni Levi,Gerald M. Edelman +5 more
TL;DR: N-CAM may play an important role not only in the determination of synaptic areas but also in Schwann cell-axon interactions during nerve regeneration, as suggested by results of an in vivo preparation of frog basal lamina sheaths obtained by inducing the degeneration of both nerve and muscle fibers.
Book ChapterDOI
Cholinesterases: Tissue and Cellular Distribution of Molecular Forms and Their Physiological Regulation
J.-P. Toutant,Jean Massoulié +1 more
TL;DR: It is shown that the two enzymes present globular forms which exist as non-hydrophobic as well as amphiphilic molecules, and asymmetric, collagen-tailed molecules, which may be further subdivided according to the binding of lectins or according to their electrophoretic migrations in nondenaturing electrophoresis.
Book ChapterDOI
Biochemistry and Pathophysiology of the Molecular Forms of Cholinesterases
TL;DR: The two types of ChE (generic abbreviation for any Cholinesterase) are readily distinguished not only by their substrate specificity but also by their response to selective inhibitors.
Journal ArticleDOI
Cytotactin is involved in synaptogenesis during regeneration of the frog neuromuscular system
René-Marc Mège,Marc Nicolet,Marc Nicolet,Martine Pinçon-Raymond,Monique Murawsky,François Rieger +5 more
TL;DR: The results suggest that cytotactin plays a primordial role in synaptogenesis, at least during nerve regeneration and reinnervation in the adult neuromuscular system.
References
More filters
Journal ArticleDOI
Heparin solubilizes asymmetric acetylcholinesterase from rat neuromuscular junction.
TL;DR: The results strongly support the involvement of heparin‐like macromolecules in the in vivo immobilization of the collagen‐tailed forms of AChE to the basal lamina of the neuromuscular junction.
Journal ArticleDOI
Muscle and nerve in muscular dysgenesis in the mouse at birth: Sprouting and multiple innervation
TL;DR: If the cytological and biochemical data are compared, it seems possible to propose that mdg mdg myofibers and axons are in contact in several regions of the same myofiber, in variably mature appositions, and with a very dense multi-innervation.
Journal ArticleDOI
Acetylcholinesterase of mammalian neuromuscular junctions: presence of tailed asymmetric acetylcholinesterase in synaptic basal lamina and sarcolemma.
TL;DR: A sarcolemma-rich fraction can be isolated after subcellular fractionation of mouse intercostal muscles by sedimentation on a discontinuous sucrose gradient, which indicates the presence of a high proportion of postsynaptic membranes.
Journal ArticleDOI
Polymorphism of acetylcholinesterase and identification of new molecular forms after sedimentation analysis.
TL;DR: The results suggest complex structural interactions between catalytic and non catalytic subunits of AChE and do not simply fit the tailed asymmetric globular model of A cholinesterase with six molecular species.
Journal ArticleDOI
Denervation induced changes in subcellular pools of 16S acetylcholinesterase activity from adult mammalian skeletal muscle.
Hugo L. Fernandez,Joel R. Stiles +1 more
TL;DR: The level at which the motor nerve exerts its primary regulatory influence on adult mammalian skeletal muscle endplate 16S AChE activity appears to be within muscle cells.
Related Papers (5)
The Molecular Forms of Cholinesterase and Acetylcholinesterase in Vertebrates
J Massoulié,S Bon +1 more