Glyphosate‐based herbicide induces long‐lasting impairment in neuronal and glial differentiation
Luã Tainã Costa Reis,Marilena Raciti,Patricia Q. Rodriguez,Bertrand Joseph,Ibrahim Al Rayyes,Per Uhlén,Anna Falk,da Cunha Lima,Sandra Ceccatelli +8 more
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It is found that subtoxic concentrations of GBH, but not of GLY, induce long‐lasting impairments on the differentiation potential of NES cells, which resulted in a more immature neuronal profile associated to increased PAX6, NESTIN and DCX expression.Abstract:
Glyphosate‐based herbicides (GBH) are among the most sold pesticides in the world. There are several formulations based on the active ingredient glyphosate (GLY) used along with other chemicals to improve the absorption and penetration in plants. The final composition of commercial GBH may modify GLY toxicological profile, potentially enhancing its neurotoxic properties. The developing nervous system is particularly susceptible to insults occurring during the early phases of development, and exposure to chemicals in this period may lead to persistent impairments on neurogenesis and differentiation. The aim of this study was to evaluate the long‐lasting effects of a sub‐cytotoxic concentration, 2.5 parts per million of GBH and GLY, on the differentiation of human neuroepithelial stem cells (NES) derived from induced pluripotent stem cells (iPSC). We treated NES cells with each compound and evaluated the effects on key cellular processes, such as proliferation and differentiation in daughter cells never directly exposed to the toxicants. We found that GBH induced a more immature neuronal profile associated to increased PAX6, NESTIN and DCX expression, and a shift in the differentiation process toward glial cell fate at the expense of mature neurons, as shown by an increase in the glial markers GFAP, GLT1, GLAST and a decrease in MAP2. Such alterations were associated to dysregulation of key genes critically involved in neurogenesis, including PAX6, HES1, HES5, and DDK1. Altogether, the data indicate that subtoxic concentrations of GBH, but not of GLY, induce long‐lasting impairments on the differentiation potential of NES cells.read more
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Glyphosate‐based herbicide induces long‐lasting impairment in neuronal and glial differentiation
Luã Tainã Costa Reis,Marilena Raciti,Patricia Q. Rodriguez,Bertrand Joseph,Ibrahim Al Rayyes,Per Uhlén,Anna Falk,da Cunha Lima,Sandra Ceccatelli +8 more
TL;DR: It is found that subtoxic concentrations of GBH, but not of GLY, induce long‐lasting impairments on the differentiation potential of NES cells, which resulted in a more immature neuronal profile associated to increased PAX6, NESTIN and DCX expression.
Journal ArticleDOI
Poisoning Regulation, Research, Health, and the Environment: The Glyphosate-Based Herbicides Case in Canada
TL;DR: In this article , the authors analyze how as a result of the scientific and regulatory captures of relevant Canadian agencies by the pesticide industry, the Canadian regulation and scientific assessment of pesticides are deficient and lagging behind other countries, using the GBH case as a basis for analysis.
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Integrated metabolomics and transcriptomics reveal glyphosate based‐herbicide induced reproductive toxicity through disturbing energy and nucleotide metabolism in mice testes
TL;DR: In this article , the toxic effects of GBH on reproduction were investigated in mice exposed to 0 (control group), 50 (low-dose group), 250 (middledose group) and 500 (high-dose) mg/kg/day GBH for 30 days.
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Pesticides as a risk factor for cognitive impairment: Natural substances are expected to become alternative measures to prevent and improve cognitive impairment
TL;DR: In this paper , the systemic relationships between the possible mechanisms of pesticides damage to the CNS causing cognitive impairment and related learning and memory pathways networks, as well as the structure-activity relationships between some natural substances (such as polyphenols and vitamins) and the improvement were summarized.
References
More filters
Journal ArticleDOI
p21 is a universal inhibitor of cyclin kinases
Yue Xiong,Gregory J. Hannon,Gregory J. Hannon,Hui Zhang,Hui Zhang,David J. Casso,David J. Casso,Ryuji Kobayashi,David Beach,David Beach +9 more
TL;DR: It is found that over expression of p21 inhibits the activity of each member of the cyclin/CDK family, and this results indicate that p21 may be a universal inhibitor of cyclin kinases.
Journal ArticleDOI
CNS stem cells express a new class of intermediate filament protein.
TL;DR: The predicted amino acid sequence of the nestin gene product shows that nestin defines a distinct sixth class of intermediate filament protein, extending a model in which transitions in intermediate filament gene expression reflect major steps in the pathway of neural differentiation.
Journal ArticleDOI
Cortical neurons arise in symmetric and asymmetric division zones and migrate through specific phases
TL;DR: It is shown here that neurons are generated in two proliferative zones by distinct patterns of division, and newborn neurons do not migrate directly to the cortex; instead, most exhibit four distinct phases of migration, including a phase of retrograde movement toward the ventricle before migration to the cortical plate.
Journal ArticleDOI
The p21 inhibitor of cyclin-dependent kinases controls DNA replication by interaction with PCNA
TL;DR: It is shown that p21 directly inhibits PCNA-dependent DNA replication in the absence of a cyclin/CDK, and blocks the ability of PCNA to activateDNA polymerase δ, the principal replicative DNA polymerase.
Journal ArticleDOI
Glyphosate: a once-in-a-century herbicide.
TL;DR: The use of this virtually ideal herbicide is now being threatened by the evolution of glyphosate-resistant weeds, and adoption of resistance management practices will be required to maintain the benefits of glyphosate technologies for future generations.