Journal ArticleDOI
Hemizona assay and teratozoospermia: increasing sperm insemination concentrations to enhance zona pellucida binding *
Daniel R. Franken,Thinus F. Kruger,Roelof Menkveld,Sergio Oehninger,Charles C. Coddington,Gary D. Hodgen +5 more
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TLDR
In this paper, the authors evaluated the zona-binding capacity of patients with abnormal sperm morphology, using standard hemizona assay (HZA) conditions and increasing sperm insemination concentration during the assay and determined the minimum concentration of motile sperm from fertile controls necessary to validate HZA results, using sperm from teratozoospermic patients versus proven fertile controls.About:
This article is published in Fertility and Sterility.The article was published on 1990-09-01. It has received 54 citations till now. The article focuses on the topics: Abnormal sperm morphology & Sperm.read more
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Journal ArticleDOI
Relevance of acrosome function in the evaluation of semen in vitro fertilizing ability
TL;DR: In this article, spontaneous acrosome loss after a 24-hour incubation in B2 medium and after induction by calcium ionophore A23187 and phorbol ester, Phorbol 12-myristate 13-acetate 4-O-methyl ether (TPA) was measured in 131 semen samples from patients undergoing an IVF attempt.
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The factors affecting sperm binding to the zona pellucida in the hemizona binding assay
Yuan-qing Yao,Wsb Yeung,P.C. Ho +2 more
TL;DR: The present study reports three factors that affect HZA: the base medium used, the protein source and the size of pipette used for removing loosely bound spermatozoa during HZA, which emphasize the importance of standardization of the protocol of the hemizona assay worldwide to be able to compare results between different laboratories.
Journal ArticleDOI
Teratozoospermia and its effect on male fertility potential.
M. Szczygiel,Maciej Kurpisz +1 more
TL;DR: Cytogenetic findings related to teratozoospermia are controversial as classical methodology of visualizing sperm chromosomes (through HOPA) may cause selection of sperm metaphases which can be obtained only from good‐penetrating spermatozoa.
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The identity of zona pellucida receptor on spermatozoa: an unresolved issue in developmental biology.
TL;DR: The latest insights in the identification of the sperm ZP receptor are reviewed to provide considerable understanding of the regulation of fertilization that will be useful for practical application in human contraception and reproductive medicine.
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Diagnostic significance of sperm-zona pellucida interaction
TL;DR: To fertilize an oocyte, spermatozoa must also be at least highly motile, as well as being capable of undergoing the acrosome reaction timely, penetrating through the oocyte investments and fusing with the oocytes plasma membrane properly.
References
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Journal ArticleDOI
Sperm morphologic features as a prognostic factor in in vitro fertilization.
Thinus F. Kruger,Roelof Menkveld,F. S. H. Stander,Carl Lombard,Jacobus P. Van der Merwe,Johannes A. van Zyl,Karen Smith +6 more
TL;DR: In this article, the authors conducted a prospective study in women with bilateral tubal damage to determine whether there is a prognostic value in the percentage normal sperm morphologic features in a human in vitro fertilization (IVF) program.
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Predictive value of abnormal sperm morphology in in vitro fertilization
Thinus F. Kruger,Anibal A. Acosta,Anibal A. Acosta,K.F. Simmons,K.F. Simmons,R.J. Swanson,R.J. Swanson,J.F. Matta,J.F. Matta,Sergio Oehninger,Sergio Oehninger +10 more
TL;DR: By evaluating sperm morphology with the proposed strict criteria, its predictive value in in vitro fertilization is enhanced.
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The hemizona assay (HZA): development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential * † ‡
Lani J. Burkman,Charles C. Coddington,Daniel R. Franken,Thinus F. Kruger,Zev Rosenwaks,Gary D. Hodgen +5 more
TL;DR: Sperm from fertile men exhibited significantly higher binding capacity to hemizonae compared with sperm from men who had fertilization failure during in vitro fertilization (IVF) treatment, demonstrating that the HZA may be a useful diagnostic tool in male infertility evaluations.
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Corrective measures and pregnancy outcome in in vitro fertilization in patients with severe sperm morphology abnormalities
Sergio Oehninger,Anibal A. Acosta,Mahmood Morshedi,Lucinda L. Veeck,R. James Swanson,Kathryn F. Simmons,Zev Rosenwaks +6 more
TL;DR: It is concluded that patients with severe sperm head abnormalities have a lower ability to establish successful pregnancies, even though fertilization may be achieved.
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Sperm/egg interaction: The specificity of human spermatozoa
TL;DR: The demonstration of a compatibility between the gametes of man and gibbon, not shared with cercopithecids, constitutes further evidence for inclusion of the Hylobatidae within the Hominoidea.