The factors affecting sperm binding to the zona pellucida in the hemizona binding assay
Yuan-qing Yao,Wsb Yeung,P.C. Ho +2 more
TLDR
The present study reports three factors that affect HZA: the base medium used, the protein source and the size of pipette used for removing loosely bound spermatozoa during HZA, which emphasize the importance of standardization of the protocol of the hemizona assay worldwide to be able to compare results between different laboratories.Abstract:
'To whom correspondence should be addressed Sperm binding to the zona pellucida is a prerequisite for fertilization. The hemizona binding assay (HZA) is commonly used to evaluate the zona-binding capacity of spermatozoa. The present study reports three factors that affect HZA. They were the base medium used, the protein source and the size of pipette used for removing loosely bound spermatozoa during HZA. The number of spermatozoa bound on the hemizona was compared between (1) Earle's balanced salt solution (EBSS) and Ham's F-10, and (2) between human serum and bovine serum albumin (BSA). Results indicated that EBSS and human serum both significantly increase the number of bound spermatozoa when compared to Ham's F-10 (P < 0.0001, paired Mest) and BSA (P < 0.05, paired Mest) respectively. Pipettes of different diameters were used to study the effect of size in removing loosely bound spermatozoa on hemizona. Data showed that the diameter of the pipette should be 2*200 mm, in order not to remove bound spermatozoa excessively. These results emphasize the importance of standardizati on of the protocol of the hemizona assay worldwide to be able to compare results between different laboratories.read more
Citations
More filters
Journal ArticleDOI
Glycodelin-A interacts with fucosyltransferase on human sperm plasma membrane to inhibit spermatozoa-zona pellucida binding.
Philip C.N. Chiu,MK Chung,Riitta Koistinen,Hannu Koistinen,Markku Seppälä,Pak Chung Ho,Ernest Hung Yu Ng,Kai-Fai Lee,William S.B. Yeung +8 more
TL;DR: Observations suggest that human sperm FUT5 is a receptor of glycodelin-A and zona pellucida proteins, and that glycodzin-A inhibits spermatozoa-zona binding by blocking the binding of sperm Fut5 to the zonapellucIDA.
Journal ArticleDOI
Cumulus oophorus-associated glycodelin-C displaces sperm-bound glycodelin-A and -F and stimulates spermatozoa-zona pellucida binding.
Philip C.N. Chiu,MK Chung,Riitta Koistinen,Hannu Koistinen,Markku Seppälä,Pak Chung Ho,Ernest Hung Yu Ng,Kai-Fai Lee,William S.B. Yeung +8 more
TL;DR: Glycodelin-C stimulated spermatozoazona pellucida binding in a dose-dependent manner, and it effectively displaced sperm-bound gly codelin-A and -F and enhances the zona binding capacity of spermatozosa passing through the cumulus oophorus.
Journal ArticleDOI
Zona-Binding Inhibitory Factor-1 from Human Follicular Fluid Is an Isoform of Glycodelin
TL;DR: Data suggest that ZIF-1 is a differentially glycosylated isoform of glycodelin that potently inhibits human sperm-egg interaction, and would provide a better understanding of the regulation of fertilization in humans.
Journal ArticleDOI
The effects of levonorgestrel on various sperm functions.
TL;DR: In this article, the effect of levonorgestrel on sperm motility, acrosome reaction, zona binding capacity, and oocyte fusion capacity of human spermatozoa was investigated.
Journal ArticleDOI
Native human zona pellucida glycoproteins: purification and binding properties
Philip C.N. Chiu,Ben S.T. Wong,Cheuk-Lun Lee,Ronald T.K. Pang,Kai-Fai Lee,Sutiman Bambang Sumitro,Satish K. Gupta,William S.B. Yeung +7 more
TL;DR: Purified human hZP2 bound to this region only in acrosome-reacted spermatozoa and induced hyperactivation, and stimulatory activity was dependent partly on N-linked glycosylation of hZp3.
References
More filters
Journal ArticleDOI
The hemizona assay (HZA): development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential * † ‡
Lani J. Burkman,Charles C. Coddington,Daniel R. Franken,Thinus F. Kruger,Zev Rosenwaks,Gary D. Hodgen +5 more
TL;DR: Sperm from fertile men exhibited significantly higher binding capacity to hemizonae compared with sperm from men who had fertilization failure during in vitro fertilization (IVF) treatment, demonstrating that the HZA may be a useful diagnostic tool in male infertility evaluations.
Journal ArticleDOI
Penetration of human spermatozoa into the human zona pellucida and the zona-free hamster egg: a study of fertile donors and infertile patients.
James W. Overstreet,James W. Overstreet,Ryuzo Yanagimachi,Ryuzo Yanagimachi,David F. Katz,David F. Katz,Keiko Hayashi,Keiko Hayashi,Frederick W. Hanson,Frederick W. Hanson +9 more
Journal ArticleDOI
Early Events in Mammalian Fertilization
TL;DR: A large number of the cells involved in the cell reprograming process secrete polypeptide A, which is a major component of phosphorous, but the structure of the substance itself is still poorly understood.
Journal ArticleDOI
Calcium-dependent binding of mouse epididymal spermatozoa to the zona pellucida
TL;DR: Sperm bound to the zona surface following preincubation with Ca 2+ were capable of fertilization in vitro when the eggs were subsequently transferred to the culture medium and it is proposed that this binding reaction represents a part of capacitation and not the acrosome reaction.
Journal ArticleDOI
Hemizona assay: assessment of sperm dysfunction and prediction of in vitro fertilization outcome
Sergio Oehninger,Charles C. Coddington,Daniel A. Franken,Richard T. Scott,Lani J. Burkman,Anibal A. Acosta,Gary D. Hodgen +6 more
TL;DR: The authors conclude that the HZA is a valuable tool for evaluating dysfunctional sperm-zona pellucida binding, with good predictive value for fertilization in vitro.