Herbicide Selection Promotes Antibiotic Resistance in Soil Microbiomes.
Hanpeng Liao,Xi Li,Qiue Yang,Yudan Bai,Peng Cui,Chang Wen,Chen Liu,Zhi Chen,Jiahuan Tang,Jiangang Che,Zhen Yu,Stefan Geisen,Shungui Zhou,Ville-Petri Friman,Yong-Guan Zhu +14 more
TLDR
In this article, the authors show that application of three widely used herbicides-glyphosate, glufosinate, and dicamba-increases the prevalence of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in soil microbiomes without clear changes in the abundance, diversity and composition of bacterial communities.Abstract:
Herbicides are one of the most widely used chemicals in agriculture. While they are known to be harmful to nontarget organisms, the effects of herbicides on the composition and functioning of soil microbial communities remain unclear. Here we show that application of three widely used herbicides-glyphosate, glufosinate, and dicamba-increase the prevalence of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in soil microbiomes without clear changes in the abundance, diversity and composition of bacterial communities. Mechanistically, these results could be explained by a positive selection for more tolerant genotypes that acquired several mutations in previously well-characterized herbicide and ARGs. Moreover, herbicide exposure increased cell membrane permeability and conjugation frequency of multidrug resistance plasmids, promoting ARG movement between bacteria. A similar pattern was found in agricultural soils across 11 provinces in China, where herbicide application, and the levels of glyphosate residues in soils, were associated with increased ARG and MGE abundances relative to herbicide-free control sites. Together, our results show that herbicide application can enrich ARGs and MGEs by changing the genetic composition of soil microbiomes, potentially contributing to the global antimicrobial resistance problem in agricultural environments.read more
Citations
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The role of emerging organic contaminants in the development of antimicrobial resistance
Izzie Alderton,Barry R. Palmer,Jack A. Heinemann,Isabelle Pattis,Louise Weaver,Maria Jesus Gutierrez-Gines,Jacqui Horswell,Louis A. Tremblay,Louis A. Tremblay +8 more
TL;DR: Antimicrobial resistance is a multi-faceted problem that requires input from all sectors, with robust strategies and policies needed to make headway with solving the issues of this important threat.
Journal ArticleDOI
Herbicide promotes the conjugative transfer of multi-resistance genes by facilitating cellular contact and plasmid transfer
Xi Li,Chang Wen,Chen Liu,Shiyun Lu,Zhongbing Xu,Qiue Yang,Zhi Chen,Hanpeng Liao,Shungui Zhou +8 more
TL;DR: A mechanistic understanding of the risk of bacterial resistance spread promoted by herbicides is provided, which elucidates a new perspective on nonantibiotic agrochemical acceleration of the HGT of ARGs.
Journal ArticleDOI
Plasmid-Mediated Transfer of Antibiotic Resistance Genes in Soil
TL;DR: The current scenario of plasmid-mediated migration and transmission of ARGs in natural environments and under different antibiotic selection pressures is reviewed, the current methods of plasmsid extraction and analysis are summarized, and the mechanism ofplasmid splice transfer using the F factor as an example is introduced.
Journal ArticleDOI
Herbicide promotes the conjugative transfer of multi-resistance genes by facilitating cellular contact and plasmid transfer.
TL;DR: In this article , the underlying mechanism associated with herbicide-promoted HGT was analyzed by detecting intracellular reactive oxygen species (ROS) production, extracellular polymeric substance composition, cell membrane integrity and proton motive force combined with genome-wide RNA sequencing.
Journal ArticleDOI
Molecular mechanisms underlying glyphosate resistance in bacteria.
TL;DR: Glyphosate is a nonselective herbicide that kills weeds and other plants competing with crops, and it is considered to be toxicologically safe for animals and humans as discussed by the authors.
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