High-speed, multi-modal, label-free imaging of pathological slices with a Bessel beam.
Chi Liu,Zhifeng Zhao,Cheng Jin,Ying Xiao,Guoqiang Gao,Hao Xie,Qionghai Dai,Hongfang Yin,Lingjie Kong +8 more
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TLDR
A high-speed, multi-modal, label-free MPM by Bessel scan-based strip mosaicking that enables full axial information acquisition at once and alleviates the demanding requirement of sample alignment is proposed.Abstract:
Optical imaging of stained pathological slices has become the gold standard for disease diagnosis. However, the procedure of sample preparation is complex and time-consuming. Multiphoton microscopy (MPM) is promising for label-free imaging, but the imaging speed is limited, especially for whole slice imaging. Here we propose a high-speed, multi-modal, label-free MPM by Bessel scan-based strip mosaicking. With a Bessel beam for excitation, the extended depth-of-focus not only enables full axial information acquisition at once, but also alleviates the demanding requirement of sample alignment. With the strip mosaicking protocol, we can save the time of frequent sample transferring. Besides, we add a closely-attached reflection mirror under the sample for enhancing epi-detection signals, and employ circularly polarized beams for recording comprehensive information. We demonstrate its application in multi-modal, label-free imaging of human gastric cancer slices and liver cancer slices, and show its potential in rapid disease diagnosis.read more
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Association of the Collagen Signature in the Tumor Microenvironment With Lymph Node Metastasis in Early Gastric Cancer
Dexin Chen,Dexin Chen,Gang Chen,Wei Jiang,Meiting Fu,Wenju Liu,Wenju Liu,Jian Sui,Jian Sui,Shuoyu Xu,Shuoyu Xu,Zhangyuanzhu Liu,Xiaoling Zheng,Xiaoling Zheng,Liangjie Chi,Liangjie Chi,Dajia Lin,Dajia Lin,Kai Li,Weisheng Chen,Ning Zuo,Jianping Lu,Jianxin Chen,Guoxin Li,Shuangmu Zhuo,Jun Yan +25 more
TL;DR: The findings suggest that the collagen signature in the tumor microenvironment is an independent indicator of LNM in EGC, and the prediction model based on this collagen signature may be useful in treatment decision making for patients with EGC.
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Improving Physical Optics Approximation Through Bessel Beam Scattering
TL;DR: In this paper, the scattering of a nondiffractive Bessel beam (BB) incident field by a perfect electric conducting circular cylinder is investigated and compared with the standard scattering solution by a plane wave.
Journal ArticleDOI
A Leaky-Wave Analysis of Resonant Bessel-Beam Launchers: Design Criteria, Practical Examples, and Potential Applicationsat Microwave and Millimeter-Wave Frequencies
TL;DR: In this article , the authors present a set of design rules applicable to any type of Resonant Bessel-beam Launcher, regardless of its specific realization, showing a typical application of the proposed design workflow, along with a possible use of the launchers in a modern context.
Journal ArticleDOI
Forward and Inverse Scattering of Metallic Objects Through Focused Bessel-Shaped Fields
TL;DR: In this article , the authors deal with both forward and inverse electromagnetic scattering of metallic objects by focused Bessel-shaped incident fields and show that their focusing capabilities may offer new effective possibilities for the detection of metallic targets which are not so far extended with respect to the probing wavelength, i.e., not satisfying the hypotheses of physical optics approximation.
Journal ArticleDOI
Design of a Trench-Assisted Optical Fiber for Generating Bessel Beams With Different Shapes
TL;DR: In this article , a trench-assisted optical fiber that can generate Bessel beams with different shapes in the fundamental mode was designed, which can also be adjusted by tuning the structure parameters on the cross-section of the designed trench assisted fiber.
References
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