High-throughput, high-frequency 3-D ultrasound for in utero analysis of embryonic mouse brain development.
TLDR
The results indicated that brain anatomy and cerebral vasculature can be imaged with this annular-array ultrasound imaging system and that quantitative analyses of segmented cerebral ventricles can be used to characterize volumetric changes associated with mouse brain development.Abstract:
With the emergence of the mouse as the predominant model system for studying mammalian brain development, in utero imaging methods are urgently required to analyze the dynamics of brain growth and patterning in mouse embryos. To address this need, we combined synthetic focusing with a high-frequency (38-MHz) annular-array ultrasound imaging system for extended depth-of-field, coded excitation for improved penetration and respiratory-gated transmit/receive. This combination allowed non-invasive in utero acquisition of motion-free 3-D data from individual embryos in approximately 2 min, and data from four or more embryos in a pregnant mouse in less than 30 min. Data were acquired from 148 embryos spanning 5 d of early to mid-gestational stages of brain development. The results indicated that brain anatomy and cerebral vasculature can be imaged with this system and that quantitative analyses of segmented cerebral ventricles can be used to characterize volumetric changes associated with mouse brain development.read more
Citations
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Journal ArticleDOI
Preclinical Ultrasound Imaging-A Review of Techniques and Imaging Applications
Carmel M. Moran,Adrian Thomson +1 more
TL;DR: Commercial high frequency ultrasound scanners have enabled longitudinal studies of disease progression in small animal models to be undertaken and adult, neonatal and embryonic rats, mice and zebrafish can now be scanned with resolutions down to 30 microns and with sufficient temporal resolution to enable cardiac abnormalities in all these species to be identified.
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Ultrasound-guided spectral photoacoustic imaging of hemoglobin oxygenation during development.
TL;DR: Spectral photoacoustic imaging is implemented to estimate tissue oxygenation longitudinally in pregnant mice and is used to estimate hemoglobin oxygen saturation within intact, in vivo mouse concepti from developmental day 8.5 to E16.5-a first step towards functional imaging of the maternal-fetal environment.
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Nested Graph Cut for Automatic Segmentation of High-Frequency Ultrasound Images of the Mouse Embryo
TL;DR: A fully automatic segmentation method called nested graph cut to segment images that contain multiple objects with a nested structure that can work well for nested objects without requiring manual selection of initial seeds, even if different objects have similar intensity distributions and some object boundaries are missing.
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Cardiovascular Imaging in Mice
TL;DR: This review details commonly used imaging approaches, with a focus on echocardiography and magnetic resonance imaging and brief overviews of other imaging modalities.
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Comprehensive backscattering characteristics analysis for quantitative ultrasound with an annular array: a basic study on homogeneous scattering phantom
Takeru Mizoguchi,Kazuki Tamura,Jonathan Mamou,Jeffrey A. Ketterling,Kenji Yoshida,Tadashi Yamaguchi +5 more
TL;DR: This study seeks to improve the accuracy of HFU, QUS-based parameters on the envelope statistics and frequency-based analysis by using an annular array that allows for an extended DOF and demonstrated that the annular arrays provides a stable analysis over an extended axial range.
References
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Book
The Atlas of Mouse Development
TL;DR: Assessment of Developmental Stage of Pre- and Postimplantation Mouse Embryos Based on the Staging System of Theiler (1989) and general Observations on the Urogenital Ridges, Gonads, and Genital Duct System.
Journal ArticleDOI
The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain
Andrew P. McMahon,Allan Bradley +1 more
TL;DR: The Wnt-1 (int-1) proto-oncogene, which encodes a putative signaling molecule, is expressed exclusively in the developing central nervous system and adult testes and its normal role is in determination or subsequent development of a specific region of thecentral nervous system.
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Targeted disruption of the murine int-1 proto-oncogene resulting in severe abnormalities in midbrain and cerebellar development.
Kirk R. Thomas,Mario R. Capecchi +1 more
TL;DR: The function of int–1 in the mouse is explored by disrupting one of the two int-1 alleles in mouse embryo-derived stem cells using positive–negative selection and generating a chimaeric mouse that transmitted the mutant allele to its progeny.
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Multiple developmental defects in Engrailed-1 mutant mice: an early mid-hindbrain deletion and patterning defects in forelimbs and sternum.
TL;DR: The results of these studies suggest a cell autonomous role for En-1 in generation and/or survival of mid-hindbrain precursor cells and also a non-cell autonomous role in signalling normal development of the limbs and possibly sternum.
Journal ArticleDOI
A mouse for all reasons.
TL;DR: Three major mouse knockout programs are underway worldwide, working together to mutate all protein-encoding genes in the mouse using a combination of gene trapping and gene targeting in mouse embryonic stem cells.