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Open AccessJournal ArticleDOI

High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5.

Raja Noor Zaliha Raja Abdul Rahman, +3 more
- 15 Jun 2005 - 
- Vol. 341, Iss: 2, pp 267-274
TLDR
The purified S5 lipase exhibited the highest activity in the presence of palm oil as a natural oil and triolein as a synthetic triglyceride and showed random positional specificity on the thin-layer chromatography.
About: 
This article is published in Analytical Biochemistry.The article was published on 2005-06-15 and is currently open access. It has received 149 citations till now. The article focuses on the topics: Lipase & Triolein.

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Citations
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Journal ArticleDOI

Organic solvent-tolerant enzymes

Noriyuki Doukyu, +1 more
- 15 Feb 2010 - 
TL;DR: Most enzymes used in these studies did not originally exhibit high activity and stability in the presence of organic solvents because they were not screened as organic solvent-tolerant enzymes, so their potential for applications in industrial chemical processes is underestimated.
Journal Article

[Organic solvent-tolerant enzymes].

Hiroyasu Ogino
- 01 Dec 2009 - 
TL;DR: The use of organic solvents as reaction media for enzymatic reactions provides numerous industrially attractive advantages compared to traditional aqueous reaction systems as mentioned in this paper, however, despite the advantages, native enzymes almost universally exhibit low activities and/or stabilities in the presence of organic solvent-tolerant enzymes.
Journal ArticleDOI

Lipase catalysis in organic solvents: advantages and applications

Ashok Kumar, +3 more
- 13 Jan 2016 - 
TL;DR: The present review describes the advantages of lipase-catalyzed reactions in organic Solvents and various effects of organic solvents on their activity.
Journal ArticleDOI

Production of biodiesel by enzymatic transesterification: review.

Abdel E. Ghaly, +3 more
- 30 Jun 2010 - 
TL;DR: A comprehensive review of the literature on the subject of biodiesel production was carried out in this paper, where the most popular microbes used for their lipases have been filamentous fungi and recombinant bacteria.
Journal ArticleDOI

Organic solvent tolerant lipases and applications.

Shivika Sharma, +1 more
- 02 Feb 2014 - 
TL;DR: The main emphasis is to study the nature of organic solvent tolerant lipases, and the potential industrial applications that make lipases the biocatalysts of choice for the present and future have been presented.
References
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Journal ArticleDOI

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli
- 15 Aug 1970 - 
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Marion M. Bradford
- 07 May 1976 - 
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article

Cleavage of structural proteins during the assemble of the head of bacterio-phage T4

U. K. Laemmli
- 01 Jan 1970 - 
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI

Improving enzymes by using them in organic solvents

Alexander M. Klibanov
- 11 Jan 2001 - 
TL;DR: The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media, and they have found numerous potential applications, some of which are already commercialized.
Journal ArticleDOI

Interactions of cyclic hydrocarbons with biological membranes.

J. Sikkema, +2 more
- 18 Mar 1994 - 
TL;DR: The impairment of microbial activity by the cyclic hydrocarbons most likely results from hydrophobic interaction with the membrane, which affects the functioning of the membrane and membrane-embedded proteins.
Related Papers (5)

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli
- 15 Aug 1970 - 

Industrial applications of microbial lipases

Fariha Hasan, +2 more
- 26 Jun 2006 - 

Bacterial lipases: an overview of production, purification and biochemical properties.

Rani Gupta, +2 more
- 14 Feb 2004 - 

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Marion M. Bradford
- 07 May 1976 - 

Protein Measurement with the Folin Phenol Reagent

Oliver H. Lowry, +3 more
- 01 Nov 1951 - 
Frequently Asked Questions (1)
Q1. What have the authors contributed in "High-yield purification of an organic solvent-tolerant lipase from pseudomonas sp. strain s5 abstract an organic solvent-tolerant s5 lipase was purified by affinity chromatography and anion" ?

An organic solvent-tolerant S5 lipase was purified by affinity chromatography and anion exchange chromatography this paper.