Journal ArticleDOI
Higher environmental temperature-induced change in synaptosomal acetylcholinesterase activity of brain regions.
TLDR
The results suggests that heat exposure increases the lipid fluidity of synaptosomal membrane depending on the brain region which may expose the catalytic site of the enzyme (AChE) and hence activate the synaptoomal membrane bound AChE activity in brain regions.Abstract:
Exposure of adult male albino rats to higher environmental temperature (HET) at 35 degrees for 2-12 hr or at 45 degrees for 1-2 hr increases hypothalamic synaptosomal acetylcholinesterase (AChE) activity. Synaptosomal AChE activity in cerebral cortex of rats exposed to 35 degrees for 12 hr and in cerebral cortex and pons-medulla of rats exposed to 45 degrees for 1-2 hr are also activated. AChE activity of synaptosomes prepared from normal rat brain regions incubated in-vitro at 39 degrees or 41 degrees for 0.5 hr increases significantly in cerebral cortex and hypothalamus. The activation of AChE in pons-medulla is also observed when this brain region is incubated at 41 degrees for 0.5 hr. Increase of (a) the duration of incubation at 41 degrees and (b) the incubation temperature to 43 degrees under in-vitro condition decreases the synaptosomal AChE activity. Lineweaver-Burk plots indicate that (a) in-vivo and in-vitro HET-induced increases of brain regional synaptosomal AChE activity are coupled with an increase of Vmax without any change in Km (b) very high temperature (43 degrees under in-vitro condition) causes a decrease in Vmax with an increase in Km of AChE activity irrespective of brain regions. Arrhenius plots show that there is a decrease in transition temperature in hypothalamus of rats exposed to either 35 degrees or 45 degrees; whereas such a decrease in transition temperature of the pons-medulla and cerebral cortex regions are observed only after exposure to 45 degrees.(ABSTRACT TRUNCATED AT 250 WORDS)read more
Citations
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Journal ArticleDOI
Effects of Temperature in Juvenile Seabass ( Dicentrarchus labrax L.) Biomarker Responses and Behaviour: Implications for Environmental Monitoring
TL;DR: The effects of temperature on European seabass (Dicentrarchus labrax L.) juveniles were investigated using a 30-day bioassay carried out at 18 and 25°C in laboratory conditions as discussed by the authors.
Journal Article
High and low temperatures affect rat hippocampal synaptosome’s viability and functions
Sahand Asharfpour,Fereshteh Pourabdolhossein,Forough Ebrahim Tabar,Manouchehr Ashrafpour,Mojdeh Navidhamidi,Sima Shahabi,Maryam Ghasemi-Kasman,Alireza Asgari +7 more
TL;DR: Assessment of synaptosome viability and function at 37, 4°C and room temperature (RT) during 6 hours after its extraction of male Wistar rats found thatsynaptosomes preserved their viability andfunction at RT, 37 and4°C at least for 3 hours after extraction and reduced over time.
References
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Journal Article
Effects of cannabinoids on catecholamine uptake and release in hypothalamic and striatal synaptosomes.
M K Poddar,W L Dewey +1 more
TL;DR: Results demonstrate that delta 9- and delta 8-THC, but not CBN and CBD, produce a biphasic effect on the uptake and release of DA and NE in the corpus striatum and hypothalamic regions of brain and delta 4-Tetrahydrocannabinol is more potent than delta 9 -THC on both uptake and releases.
Journal ArticleDOI
Lipid dependence of activity-temperature relationship of (Na+, K+)-activated ATPase.
Ryo Tanaka,Akira Teruya +1 more
TL;DR: The effects of temperature effects on lipid moieties of (Na + , K + )-dependent ATPases of bovine cerebral cortex and bullfrog kidney were found to depend on the lipid moiety, and to be constant with a given lipid regardless of the enzyme source.
Journal ArticleDOI
The role of tightly bound phospholipid in the activity of erythrocyte acetylcholinesterase
TL;DR: It is concluded that the physical state of cardiolipin, tightly bound to acetylcholinesterase by ionic interactions, modulates the catalytic activity of acetyl cholinestersterase.
Journal ArticleDOI
Use of Arrhenius plots of Na-K ATPase and acetylcholinesterase as a tool for studying changes in lipid-protein interactions in neuronal membranes during brain development.
M. Nemat‐Gorgani,E. Meisami +1 more
TL;DR: Compared to acetylcholinesterase, Na‐K ATPase is more strongly affected by the rise in temperature and that this response is further enhanced with age, which is discussed in terms of the differences and changes in the interactions of Na‐ K ATPase and acetyl cholinestersterase with membrane lipids during the postnatal phase of brain development.
Journal ArticleDOI
Carbaryl-induced elevation of corticosterone level and cholinergic mechanism
Subir K. Ray,Mrinal K. Poddar +1 more
TL;DR: Results suggest that the carbaryl-induced rise in the corticosterone level in the adrenal gland and plasma is not due to a cholinergic mechanism.
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