Hydrogel and neural progenitor cell delivery supports organotypic fetal spinal cord development in an ex vivo model of prenatal spina bifida repair.
Juan C. Biancotti,Kendal A. Walker,Guihua Jiang,Julie Di Bernardo,Lonnie D. Shea,Shaun M. Kunisaki +5 more
TLDR
The biocompatibility of fibrin hydrogel patches on the fetal spinal cord is demonstrated and this organotypic slice culture system is suggested as a useful platform for evaluating mechanisms of damage and repair in children with neural tube defects.Abstract:
Studying how the fetal spinal cord regenerates in an ex vivo model of spina bifida repair may provide insights into the development of new tissue engineering treatment strategies to better optimize...read more
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Multifunctional GelMA platforms with nanomaterials for advanced tissue therapeutics.
Amal George Kurian,Hae-Won Kim,Rajendra K. Singh,Kapil D. Patel,Kapil D. Patel,Jung-Hwan Lee,Hae-Won Kim +6 more
TL;DR: Recent efforts in nanomaterials-combined GelMA hydrogels that are considered as next-generation multifunctional platforms for tissue therapeutics are systematically reviewed.
Journal ArticleDOI
Multifunctional GelMA platforms with nanomaterials for advanced tissue therapeutics
TL;DR: In this article , a review of nanomaterials-combined GelMA hydrogels that are considered as next-generation multifunctional platforms for tissue therapeutics is presented.
Local gene delivery from Ecm coated Poly (lactide co glycolide) multiple channel bridges after spinal cord injury
TL;DR: In vivo and in vitro studies revealed that fibronectin produced greater expression levels and immobilization efficiencies compared with collagen, laminin, and no coating, and lipoplex incubation on ECM-coated PLG increased expression relative to either of the drying methods.
Journal ArticleDOI
Human Mesenchymal Stem Cell-Derived Extracellular Vesicles Promote Neural Differentiation of Neural Progenitor Cells
Soyeon Park,Da-Seul Kim,Hyun Mun Kim,Jun-Kyu Lee,Dong-Youn Hwang,Tae-Hyung Kim,Seung-Suk You,D.K. Han +7 more
TL;DR: dEVs promoted neuronal differentiation of neural progenitor cells in vitro, suggesting that dEVs are a prospective candidate for EV-based neurological disorder regeneration therapy.
Targeted Quantitative Amniotic Cell Profiling: A Potential Diagnostic Tool In the Prenatal Management of Neural Tube Defects
TL;DR: In this article, the proportions of neural and mesenchymal stem cells in the amniotic fluid correlate with the type and size of experimental neural tube defect (NTD) type and/or size.
References
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Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling
Stuart M. Chambers,Christopher A. Fasano,Eirini P. Papapetrou,Mark J. Tomishima,Michel Sadelain,Lorenz Studer +5 more
TL;DR: Noggin/SB431542-based neural induction should facilitate the use of hES and hiPS cells in regenerative medicine and disease modeling and obviate the need for protocols based on stromal feeders or embryoid bodies.
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A simple method for organotypic cultures of nervous tissue
TL;DR: Hippocampal slices prepared from 2-23-day-old neonates were maintained in culture at the interface between air and a culture medium and yielded thin slices which remain 1-4 cell layers thick and are characterized by a well preserved organotypic organization.
Journal Article
Quantification of histochemical staining by color deconvolution
TL;DR: This image analysis algorithm provides a robust and flexible method for objective immunohistochemical analysis of samples stained with up to three different stains using a laboratory microscope, standard RGB camera setup and the public domain program NIH Image.
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A Randomized Trial of Prenatal versus Postnatal Repair of Myelomeningocele
N. Scott Adzick,Elizabeth Thom,Catherine Y. Spong,John W. Brock,Pamela K. Burrows,Mark P. Johnson,Lori J. Howell,Jody A. Farrell,Mary E. Dabrowiak,Leslie N. Sutton,Nalin Gupta,Noel Tulipan,Diana L. Farmer +12 more
TL;DR: Prenatal surgery for myelomeningocele reduced the need for shunting and improved motor outcomes at 30 months but was associated with maternal and fetal risks and increased risk of preterm delivery and uterine dehiscence at delivery.
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Neural stem cells constitutively secrete neurotrophic factors and promote extensive host axonal growth after spinal cord injury
TL;DR: Grafted NSCs were genetically modified to produce neurotrophin-3, which significantly expanded NSC effects on host axons and confirmed that grafted stem cells expressed neurotrophic factor genes in vivo.