Journal ArticleDOI
Hydroxynitrile lyase from Passiflora edulis: Purification, characteristics and application in asymmetric synthesis of (R)-mandelonitrile
TLDR
The PeHNL from rinds had been isolated for the first time and the enzyme showed great ability in transcyanation of (R)-mandelonitrile with high e.e. in DBE as the co-organic solvent in a biphasic system.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2010-05-05. It has received 44 citations till now. The article focuses on the topics: Enantiomeric excess & Mandelonitrile.read more
Citations
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Journal ArticleDOI
C−C Bond-Forming Lyases in Organic Synthesis
Journal ArticleDOI
Biocatalytic Methods for C ? C Bond Formation
TL;DR: Novel enzymatic CC bond formation reactions have been applied, implying the most important benefit of biocatalysis, namely the high selectivity.
Journal ArticleDOI
Hydroxynitrile Lyases: Insights into Biochemistry, Discovery, and Engineering
TL;DR: Characteristics of many S- and R-selective HNLs with comparative tables for several enzymatic properties under biochem... will be presented.
Journal ArticleDOI
The past decade findings related with nutritional composition, bioactive molecules and biotechnological applications of Passiflora spp. (passion fruit)
Rúbia Carvalho Gomes Corrêa,Rúbia Carvalho Gomes Corrêa,Rosane Marina Peralta,Charles Windson Isidoro Haminiuk,Giselle Maria Maciel,Adelar Bracht,Isabel C.F.R. Ferreira +6 more
TL;DR: In this article, a series of emerging technologies firmed on non-thermal principals, along with encapsulation by nanoparticles methods, have been studied for the sustainable recovery of some target high added-value compounds from Passiflora spp. and their co-products, which include biologically active components such as polyphenols, terpenes, peptides, polysaccharides and dietary fibers.
Journal ArticleDOI
Synthesis of (R)-β-nitro alcohols catalyzed by R-selective hydroxynitrile lyase from Arabidopsis thaliana in the aqueous–organic biphasic system
Ken-ichi Fuhshuku,Yasuhisa Asano +1 more
TL;DR: This is the first example of the R-HNL-catalyzed synthesis of (R)-β-nitro alcohols, and the organic solvent giving the highest enantioselectivity was n-butyl acetate (AcOBu).
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
Stereochemistry of Organic Compounds
TL;DR: Stereochemistry of Alkenes Conformation of Acyclic Molecules Configuration and conformation of cyclic Molecule Stereoselective Synthesis Chiroptical Properties Chirality in Molecules Devoid of Chiral Centres as discussed by the authors.
Journal ArticleDOI
Improving enzymes by using them in organic solvents
TL;DR: The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media, and they have found numerous potential applications, some of which are already commercialized.
Book
Enzymes in synthetic organic chemistry
TL;DR: Part 1 general aspects: rate acceleration in enzyme-catalyzed reactions Michaelis-Menten kinetics enzyme inhibition specificity improvement or alteration of enzyme specifity enzyme stabilization and reactor configuration cofactor regeneration enzyme catalysis in organic solvents multienzyme systems and metabolic engineering rational design of new enzymatic catalysts references.