Immobilization of the Candida rugosa lipase onto a Scirpus grossus L.f. fiber as biocatalyst for biodiesel synthesis via hydrolysis-esterification
TLDR
The immobilized C. rugosa lipase yielded a higher hydrolysisesterification efficiency than that of the free lipase with the immobilized form looking promising for FAEE biodiesel production.Abstract:
This study aimed to immobilize the Candida rugosa lipase (C. rugosa lipase) on ground Scirpus grossus L.f. fibers by glutaraldehyde-crosslinking to form a hydrolysis-esterification catalyst for biodiesel synthesis. The effects of different glutaraldehyde concentrations and solvent for 3aminopropyltriethoxysilane (3-APTES) activation of the fibers on the resultant immobilized lipase activity, protein loading, degree of immobilization and catalytic efficiency were investigated. The optimum condition found was to first activate the S. grossus L.f. fibers using 2% (w/v) of 3-APTES in distilled water and subsequently crosslink with 0.2% (w/v) glutaraldehyde prior to C. rugosa lipase immobilization at pH 7. The immobilized C. rugosa lipase was then evaluated as a biocatalyst for biodiesel synthesis via the hydrolysis-esterification of palm oil and bioethanol through monitoring the production of free fatty acids (FFAs) and fatty acid ethyl ester (FAEE, biodiesel). The reusability of immobilized lipase was also determined. The immobilized C. rugosa lipase yielded a higher hydrolysisesterification efficiency (that is, FFA and FAEE formation) than that of the free lipase with the immobilized form looking promising for FAEE biodiesel production. The C. rugosa lipase immobilized with 0.2% (w/v) glutaraldehyde exhibited the highest reuse stability, retaining some hydrolysis and esterification activity for up to six uses, whereas crosslinking with higher [0.5% or 0.8% (w/v)] glutaraldehyde levels resulted in a loss of both activities within four uses.read more
Citations
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Structure and properties of oil palm-based nanocellulose reinforced chitosan nanocomposite for efficient synthesis of butyl butyrate
Nursyafiqah Elias,Sheela Chandren,Nursyafreena Attan,Naji Arafat Mahat,Fazira Ilyana Abdul Razak,Joazaizulfazli Jamalis,Roswanira Abdul Wahab +6 more
TL;DR: NMR analyses on the purified butyl butyrate confirmed that the ester was successfully synthesized and its suitability as nano-fillers for preparing the chitosan/nanocellulose (CS-NC) supports to immobilize Candida rugosa lipase (CRL) to produce the CRL/ CS-NC biocatalysts.
Journal ArticleDOI
Characterization, optimization and stability studies on Candida rugosa lipase supported on nanocellulose reinforced chitosan prepared from oil palm biomass.
Nursyafiqah Elias,Sheela Chandren,Fazira Ilyana Abdul Razak,Joazaizulfazli Jamalis,Nashi Widodo,Roswanira Abdul Wahab +5 more
TL;DR: This research provides a promising approach for expanding the use of NC from OPFL for enhancing enzyme activity in favour of an alternative eco-friendly means to synthesize butyl butyrate.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Biodiesel processing and production
TL;DR: Biodiesel is an alternative diesel fuel that is produced from vegetable oils and animal fats, which consists of the monoalkyl esters formed by a catalyzed reaction of the triglycerides in the oil or fat with a simple monohydric alcohol.
Journal ArticleDOI
Conversion of vegetable oil to biodiesel using immobilized Candida antarctica lipase
Yuji Shimada,Yomi Watanabe,Taichi Samukawa,Akio Sugihara,Hideo Noda,Hideki Fukuda,Yoshio Tominaga +6 more
TL;DR: In this paper, the authors attempted continuous methanolysis of vegetable oil by an enzymatic process, which was conducted by adding methanol stepwise to avoid lipase inactivation.
Journal ArticleDOI
Lipase-catalyzed production of biodiesel
TL;DR: Conditions were established for converting tallow to short-chain alkyl esters at more than 90% conversion and these same conditions also proved effective for transesterfying vegetable oils and high fatty acid-containing feedstocks to their respective alKYl ester derivatives.
Journal ArticleDOI
Interaction of Silane Coupling Agents with Cellulose
Mekki Abdelmouleh,Sami Boufi,Abdelhamid Ben Salah,and Mohamed Naceur Belgacem,Alessandro Gandini +4 more
TL;DR: In this article, the adsorption of prehydrolyzed alkoxysilanes onto the surface of cellulosic fibers in ethanol/water mixtures has been studied quantitatively.