Immunolocalization of estrogen receptor β in the mouse brain: Comparison with estrogen receptor α
Sudha W. Mitra,Elena Hoskin,Joel B. Yudkovitz,Lisset Pear,Hilary A. Wilkinson,Shinji Hayashi,Donald W. Pfaff,Sonoko Ogawa,Susan P. Rohrer,James M. Schaeffer,Bruce S. McEwen,Stephen E. Alves +11 more
Reads0
Chats0
TLDR
The development of an ERβ-selective antibody that cross-reacts with mouse, rat, and human ERβ protein and its use to determine the distribution of ERβ in the murine brain is reported.Abstract:
Estrogen receptor α (ERα) and ERβ are members of the steroid nuclear receptor family that modulate gene transcription in an estrogen-dependent manner. ER mRNA and protein have been detected both peripherally and in the central nervous system, with most data having come from the rat. Here we report the development of an ERβ-selective antibody that cross-reacts with mouse, rat, and human ERβ protein and its use to determine the distribution of ERβ in the murine brain. Further, a previously characterized polyclonal antibody to ERα was used to compare the distribution of the two receptors in the first comprehensive description of ER distribution specifically in the mouse brain. ERβ immunoreactivity (ir) was primarily localized to cell nuclei within select regions of the brain, including the olfactory bulb, cerebral cortex, septum, preoptic area, bed nucleus of the stria terminalis, amygdala, paraventricular hypothalamic nucleus, thalamus, ventral tegmental area, substantia nigra, dorsal raphe, locus coeruleus...read more
Citations
More filters
Journal ArticleDOI
Estrogen receptors and human disease
TL;DR: This Review will describe diseases in which estrogen plays a role in the development or severity of disease, through the ER, which serves as the basis for many therapeutic interventions.
Journal ArticleDOI
Regulation of Kiss1 gene expression in the brain of the female mouse.
TL;DR: Kiss1 gene encodes a family of neuropeptides called kisspeptins, which activate the receptor G protein-coupled receptor-54 and play a role in the neuroendocrine regulation of GnRH secretion and whether estradiol regulates KiSS-1 in the forebrain of the female mouse is examined.
Journal ArticleDOI
Differential Regulation of KiSS-1 mRNA Expression by Sex Steroids in the Brain of the Male Mouse
Jeremy Troy Smith,Heather M. Dungan,Elizabeth A. Stoll,Michelle L. Gottsch,Robert E. Braun,Stephen M. Eacker,Donald K. Clifton,Robert A. Steiner +7 more
TL;DR: Kisspeptins are products of the Kiss1 gene, which bind to GPR54, a G protein-coupled receptor, and the effects of T are mediated by both ERalpha and AR pathways, suggesting that both estrogen receptor (ER) and androgen receptor (AR) play a role in T-mediated regulation of KiSS-1.
Journal ArticleDOI
Estrogen Actions in the Brain and the Basis for Differential Action in Men and Women: A Case for Sex-Specific Medicines
Glenda Gillies,Simon McArthur +1 more
TL;DR: This review focuses on sex dimorphisms in the ability of estradiol to influence synaptic plasticity, neurotransmission, neurodegeneration, and cognition, which, it is argued, are due in a large part to sex differences in the organization of the underlying circuitry.
Journal ArticleDOI
Estrogen synthesis and signaling pathways during aging: from periphery to brain
Jie Cui,Yong Shen,Rena Li +2 more
TL;DR: This comprehensive review provides new insights into estrogens by giving a better understanding of the tissue-specific estrogen effects and their roles in various diseases.
References
More filters
Book
The Mouse Brain in Stereotaxic Coordinates
TL;DR: The 3rd edition of this atlas is now in more practical 14"x11" format for convenient lab use and includes a CD of all plates and diagrams, as well as Adobe Illustrator files of the diagrams, and a variety of additional useful material.
Journal ArticleDOI
Cloning of a novel receptor expressed in rat prostate and ovary.
TL;DR: It is concluded that clone 29 cDNA encodes a novel rat ER, which is suggested be named rat ERbeta to distinguish it from the previously cloned ER (ERalpha) from rat uterus.
Journal ArticleDOI
Comparative distribution of estrogen receptor-alpha and -beta mRNA in the rat central nervous system.
TL;DR: Comparing the distribution of the classical and novel forms of ER mRNA‐expressing neurons in the central nervous system (CNS) of the rat with in situ hybridization histochemistry provides evidence that the region‐specific expression of ER‐α, ER‐β, or both may be important in determining the physiological responses of neuronal populations to estrogen action.
PatentDOI
DIFFERENTIAL LIGAND ACTIVATION OF ESTROGEN RECEPTORS ER$g(a) AND ER$g(b) AT AP1 SITES
Peter J. Kushner,Jan-Ake Gustafsson,George G.J.M. Kuiper,Stefan K. Nilsson,Kolja Paech,Thomas S. Scanlan,Paul W. Webb +6 more
TL;DR: In this article, a cell comprising an estrogen receptor beta (ER beta ), AP1 proteins, and a construct comprising a promoter comprising an AP1 site which regulates expression of a first reporter gene is contacted with the test compound and changes in expression levels of the reporter gene are detected indicating whether the test compounds activate transcription, inactivate transcription or have no effect at the AP1 sites.
Journal ArticleDOI
Distribution of androgen and estrogen receptor mRNA‐containing cells in the rat brain: An in situ hybridization study
TL;DR: AR and ER may modulate nonolfactory sensory information as well since labeled cells were found in regions involved in the central relay of somatosensory information, including the mesencephalic nucleus of the trigeminal nerve, the ventral thalamic nuclear group, and the dorsal horn of the spinal cord.