Journal ArticleDOI
Immunoprotective Efficacy of a Purified 39 kDa Nymphal Antigen of Hyalomma anatolicum anatolicum
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TLDR
Soluble nymphal antigens (HNAg) were purified by immunoaffinity chromatography using CNBr-activated Sepharose 4B coupled with immunoglobulin ligands from animals immunized with HNAg and 69–71% protected against challenge infestations, and 8% recovery of the purified protein was obtained.Abstract:
Soluble nymphal antigens (HNAg) were purified by immunoaffinity chromatography using CNBr-activated Sepharose 4B coupled with immunoglobulin ligands from animals immunized with HNAg and 69–71% protected against challenge infestations, and 8% recovery of the purified protein (Aff-HNAg) was obtained. Following immunization of crossbred calves (Bos indicus×Bos taurus) with 1600 μg of Aff-HNAg in three divided doses, significant rejections of larvae (p<0.001, 84.2%), nymphs (p<0.05, 61.4%) and adults (p<0.05, 58.7%) were recorded. No significant changes were recorded in the engorgement weights of the larvae and nymphs, but there was a significant (p<0.05) reduction in the weight of the engorged adults. Immunization conferred a significant decrease in the numbers of resultant nymphs (p<0.001) and adults (p<0.001) that had fed on the immunized animals. SDS-PAGE analysis identified a 39 kDa protein, previously isolated from larvae of Hyalomma anatolicum anatolicum, as the antigen responsible for the induction of resistance against all the stages of the tick.read more
Citations
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Anti-tick vaccines.
TL;DR: There is now abundant evidence that vaccination with defined protein antigens is able to induce significant immunity to tick infestation, but under most circumstances the use of a tick vaccine as the single, stand alone control technology is likely to require more efficacious vaccines than those currently available.
Journal ArticleDOI
Control of ticks of ruminants, with special emphasis on livestock farming systems in India: present and future possibilities for integrated control--a review.
TL;DR: The problems and opportunities for the integrated control of ticks of ruminants with special emphasis on livestock farming systems in India are reviewed.
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Problem of ticks and tick-borne diseases in India with special emphasis on progress in tick control research: a review.
Srikant Ghosh,Gaurav Nagar +1 more
TL;DR: There is a need to reassess the renewed threat posed by the tick vectors and to prioritize the tick control research programme, with emphasis on acaricide resistance, tick vaccine and the development of potential phytoacaricides as an integral part of integrated tick control programme.
Journal ArticleDOI
The molecular revolution in the development of vaccines against ectoparasites.
TL;DR: The application of a spectrum of molecular techniques has begun to revolutionise the authors' understanding of protective immune responses to ectoparasites and the targets for those responses, and this should contribute to the eventual development of a range of recombinant vaccines.
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Book
Antibodies: A Laboratory Manual
Ed Harlow,David P. Lane +1 more
TL;DR: A second edition of Antibodies: A Laboratory Manual is being published in September 2013, Revised, extended and updated by Edward Greenfield of the Dana-Farber Cancer Center, the material has been recast with extensive new information and new chapters have been added.