Journal ArticleDOI
Improvements in immunostaining samples embedded in methacrylate: localization of microtubules and other antigens throughout developing organs in plants of diverse taxa.
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TLDR
Using this method, it is found that interphase meristematic cells in all of these species have microtubules not only in the usual cortical array but also throughout their cytoplasm.Abstract:
Microtubules are important in plant growth and development. Localizing microtubules in sectioned material is advantageous because it allows any tissue of interest to be studied and it permits the positional relations of the cells within the organ to be known. We describe here a method that uses semi-thin (0.5-2 μm) sections of material embedded in butyl-methylmethacrylate, to which 10 mM dithiothreitol was added. After removing the embedding material and using indirect immunofluorescence staining, we obtain clear images of microtubules, actin microfilaments, callose and pulse-fed bromodeoxyuridine. This method works on the root tissues of Arabidopsis thaliana(L.) Heynh, Pinus radiataD. Don, Zamia furfuraceaAit., Azolla pinnataR. Br. and on sporophytic tissues of Funaria hygrometricaHedw. In general, most of the cells in the organs studied are successfully stained. Using this method, we find that interphase meristematic cells in all of these species have microtubules not only in the usual cortical array but also throughout their cytoplasm. The presence of the calcium chelator ethylene glycol-bis(β-aminoethyl ether)N,N,N',N'-tetraacetic acid EGTA in fixation buffers led to some tissue damage, and did not enhance the preservation of microtubules. The common assumption that EGTA-containing buffers stabilize plant microtubules during fixation appears unwarranted.read more
Citations
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Journal ArticleDOI
Formation of lateral root meristems is a two-stage process.
TL;DR: It is hypothesized that meristem formation is a two-step process involving an initial period during which a population of rapidly dividing, approximately isodiametric cells that constitutes the primordium is formed, and a subsequent stage during which meristen organization takes place within thePrimordium.
Journal ArticleDOI
Embedding of Bone Samples in Methylmethacrylate: An Improved Method Suitable for Bone Histomorphometry, Histochemistry, and Immunohistochemistry
TL;DR: The modified MMA embedding process, using commercially available methacrylates can be used without purification, and the histologic quality of bone sections is comparable to that of conventionally MMA-embedded bone specimens.
Journal ArticleDOI
Expression of the plant cyclin‐dependent kinase inhibitor ICK1 affects cell division, plant growth and morphology
TL;DR: It is demonstrated that ICK1 acts as a CDK inhibitor in the plant, and the inhibition of cell division byICK1 expression has profound effects on plant growth and development, and it is suggested that alterations of plant organ shape can be achieved by restriction ofcell division.
Journal ArticleDOI
The Arabidopsis thaliana AtSUC2 Gene is Specifically Expressed in Companion Cells
Ruth Stadler,Norbert Sauer +1 more
TL;DR: The presented data indicate that phloem loading in Arabidopsis may be catalyzed by the AtSUC2 sucrose carrier which transports sucrose into the companion cells.
Journal ArticleDOI
New techniques enable comparative analysis of microtubule orientation, wall texture, and growth rate in intact roots of Arabidopsis.
TL;DR: This study developed methods to examine cellulose microfibrils by field emission scanning electron microscopy and microtubules by immunofluorescence in essentially intact roots and observed microtubule-microfibril alignment discord before cells enter their elongation phase.
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Journal ArticleDOI
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Kiyotaka Okada,Yoshiro Shimura +1 more
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Journal ArticleDOI
Two monoclonal antibodies to actin: one muscle selective and one generally reactive.
TL;DR: Two IgG1, kappa monoclonal antibodies against actin have been obtained from a fusion in which chicken gizzard actin was used as the immunogen, and their epitopes appear to overlap.
Journal ArticleDOI
Structure of cortical microtubule arrays in plant cells
AR Hardham,BE Gunning +1 more
TL;DR: The distribution of C-shaped terminations provides evidence for at least local polarity in the arrays, along with implications for the development, maintenance, and orientation of the arrays and their possible relationship to the orientation of cellulose deposition.
Journal ArticleDOI
Immunofluorescence microscopy of organized microtubule arrays in structurally stabilized meristematic plant cells.
TL;DR: The retention of antigenicity in permeabilized, isolated, stabilized cells from typical, wall-enclosed plant cells has much potential for plant immunocytochemistry, and in particular should facilitate work on the role of microtubules in the morphogenesis of organized plant tissues.