Journal ArticleDOI
In situ identification and N2 and C fixation rates of uncultivated cyanobacteria populations
Andreas Krupke,Niculina Musat,Julie LaRoche,Wiebke Mohr,Bernhard M. Fuchs,Rudolf Amann,Marcel M. M. Kuypers,Rachel A. Foster +7 more
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TLDR
Two common co-occurring unicellular groups are identified and quantified and their cellular activities are measured by nanoSIMS, a globally important process often mediated by diazotrophic cyanobacteria in the open ocean.About:
This article is published in Systematic and Applied Microbiology.The article was published on 2013-06-01. It has received 69 citations till now. The article focuses on the topics: Crocosphaera watsonii & Cape verde.read more
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The small unicellular diazotrophic symbiont, UCYN-A, is a key player in the marine nitrogen cycle
Clara Martínez-Pérez,Wiebke Mohr,Carolin R. Löscher,Julien Dekaezemacker,Sten Littmann,Pelin Yilmaz,Nadine Lehnen,Bernhard M. Fuchs,Gaute Lavik,Ruth A. Schmitz,Julie LaRoche,Marcel M. M. Kuypers +11 more
TL;DR: The analysis of global 16S rRNA gene databases showed that UCYN-A occurs in surface waters from the Arctic to the Antarctic Circle and thus probably contributes to N2 fixation in a much larger oceanic area than previously thought.
Journal ArticleDOI
NanoSIMS for biological applications: Current practices and analyses
TL;DR: The authors describe the major experimental factors that should be considered prior to NanoSIMS analysis and then provide information on best practices for data analysis and image generation, which includes an in-depth discussion of appropriate colormaps.
Journal ArticleDOI
Genetic diversity of the unicellular nitrogen-fixing cyanobacteria UCYN-A and its prymnesiophyte host.
Anne W. Thompson,Brandon J. Carter,Kendra A. Turk-Kubo,Francesca Malfatti,Farooq Azam,Jonathan P. Zehr +5 more
TL;DR: The relevance of UCYN-A's genetic diversity to its symbiosis and ecology was explored through combining flow cytometric cell sorting and molecular techniques to determine the host identity, nifH expression patterns and host cell size of one newly discovered clade, UCyn-A2, at a coastal site.
Journal ArticleDOI
Symbiotic unicellular cyanobacteria fix nitrogen in the Arctic Ocean.
Katie Harding,Kendra A. Turk-Kubo,Rachel E. Sipler,Matthew M. Mills,Deborah A. Bronk,Jonathan P. Zehr +5 more
TL;DR: It is reported that the UCYN-A symbiosis is present and fixing N2 in the Western Arctic and Bering Seas, further north than any previously reported N2-fixing marine cyanobacteria, and results show definitively that cyanobacterial N2 fixation is not constrained to subtropical waters, challenging paradigms and models of global N1 fixation.
Journal ArticleDOI
Tracking microbial interactions with NanoSIMS.
TL;DR: Recent applications of nanoSIP, particularly where used to track exchanges of isotopically labelled molecules between organisms, are highlighted, including metabolic interactions within syntrophic consortia, carbon/nitrogen fluxes between phototrophs and their heterotrophic partners, and symbiont-host nutrient sharing.
References
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Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.
TL;DR: Fluorescent oligonucleotide hybridization probes were used to label bacterial cells for analysis by flow cytometry and the intensity of fluorescence was increased additively by the combined use of two or three fluorescent probes complementary to different regions of the same 16S rRNA.
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The Domain-specific Probe EUB338 is Insufficient for the Detection of all Bacteria: Development and Evaluation of a more Comprehensive Probe Set
TL;DR: Two supplementary versions of probe EUB338 are designed and evaluated for in situ detection of most of those phyla not detected with this probe, which should allow a more accurate quantification of members of the domain Bacteria in future molecular ecological studies.
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Nitrogen, Phosphorus, and Eutrophication in the Coastal Marine Environment
TL;DR: Removal of phosphate from detergents is not likely to slow the eutrophication of coastal marine waters, and its replacement with nitrogen-containing nitrilotriacetic acid may worsen the situation.
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Nutrient limitation of phytoplankton in freshwater and marine environments: A review of recent evidence on the effects of enrichment1
R. E. Hecky,Peter Kilham +1 more
TL;DR: It is concluded that the extent and severity of N limitation in the marine environment remain an open question, despite the fact that by the late seventies the evidence for P limitation had become so great that phosphorus control was recommended as the legislated basis for controlling eutrophication in North American and European inland waters.
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Trichodesmium, a Globally Significant Marine Cyanobacterium
TL;DR: N2 fixation by Trichodesmium is likely a major input to the marine and global nitrogen cycle.