scispace - formally typeset
Journal ArticleDOI

In vitro interaction of 1-anilino 8-naphtalene sulfonate with excitable membranes isolated from the electric organ of Electrophorus electricus

Reads0
Chats0
TLDR
In vitro binding of 1-anilino 8-naphtalene sulfonate to membrane fragments derived from the innervated, excitable, surface of electroplax cells is accompanied by a strong enhancement of fluorescence intensity and polarization and is insensitive to changes of solvent viscosity upon addition of sucrose.
About
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1969-08-07. It has received 55 citations till now. The article focuses on the topics: Electrophorus & Fluorescence anisotropy.

read more

Citations
More filters
Journal ArticleDOI

Muscle and neuronal nicotinic acetylcholine receptors

TL;DR: Nicotinic acetylcholine receptors (nAChRs) as mentioned in this paper are integral membrane proteins and prototypic members of the ligand-gated ion-channel superfamily, which has precursors in the prokaryotic world.
Journal ArticleDOI

On the process of cellular division in Escherichia coli. 3. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation.

TL;DR: The characteristics of a temperature conditional mutant of Escherichia coli K12 indicate that at high temperature, it can complete a DNA cycle already started, but not initiate a new one.
Journal ArticleDOI

In Vitro excitation of purified membrane fragments by cholinergic agonists : I. Pharmalogical properties of the excitable membrane fragments.

TL;DR: The dose-response curve and the binding curve superimpose almost exactly; in other words, the “apparent” affinity of Deca coincides with its “real’ affinity.
References
More filters
Journal ArticleDOI

The interaction of a naphthalene dye with apomyoglobin and apohemoglobin: A fluorescent probe of non-polar binding sites

TL;DR: In this paper, anilino-8-naphthalene sulfonate (ANS) was used as a fluorescent probe of non-polar regions in proteins and its fluorescence changes markedly when it is bound to the apoprotein.
Journal ArticleDOI

Fluorescence Spectroscopy of Proteins

TL;DR: Fluorescence spectroscopy of proteins, analyzing polarity, distances between groups, flexibility and conformational transitions, and its applications in medicine and materials science.
Book ChapterDOI

Rotational Brownian motion and polarization of the fluorescence of solutions.

TL;DR: The chapter discusses the rotational Brownian motion and polarization of the fluorescence of solutions, which involves the translation of the molecules but also their rotational movements.
Journal ArticleDOI

The interaction of 1-anilino-8-naphthalene sulphonate with erythrocyte membranes.

TL;DR: The present communication reports part of the programme for elucidating the nature of the interaction of ANS with membranes, since erythrocyte membranes have been widely studied with respect to structure and function they were used in this work.
Related Papers (5)