Journal ArticleDOI
The interaction of a naphthalene dye with apomyoglobin and apohemoglobin: A fluorescent probe of non-polar binding sites
TLDR
In this paper, anilino-8-naphthalene sulfonate (ANS) was used as a fluorescent probe of non-polar regions in proteins and its fluorescence changes markedly when it is bound to the apoprotein.About:
This article is published in Journal of Molecular Biology.The article was published on 1965-09-01. It has received 1420 citations till now. The article focuses on the topics: Myoglobin & Optical rotatory dispersion.read more
Citations
More filters
Journal ArticleDOI
Removal of Fatty Acids from Serum Albumin by Charcoal Treatment
TL;DR: Fluorescence spectra of human serum albumin samples indicated that impurities are sometimes present which can be removed by charcoal at neutral pH, and acid-charcoal treatment is a much more rapid method of removing lipid impurities than other methods previously described.
Journal ArticleDOI
Study of the "molten globule" intermediate state in protein folding by a hydrophobic fluorescent probe.
Gennady V. Semisotnov,N. A. Rodionova,O. I. Razgulyaev,Vladimir N. Uversky,A. F. Gripas,R. I. Gilmanshin +5 more
TL;DR: Binding of the hydrophobia fluorescent probe, 1‐anilino‐naphthalene‐8‐sulfonate (ANS), to synthetic polypeptides and proteins with a different structural organization has been studied and it has been shown that ANS has a much stronger affinity to the protein “molten globule” state.
Book ChapterDOI
Molten globule and protein folding.
TL;DR: It is predicted and confirmed experimentally that the molten globule state can exist in a living cell and plays an important role in a number of physiological processes.
Journal ArticleDOI
Gaucher Disease Glucocerebrosidase and α-Synuclein Form a Bidirectional Pathogenic Loop in Synucleinopathies
Joseph R. Mazzulli,You Hai Xu,You Hai Xu,Ying Sun,Ying Sun,Adam L. Knight,Pamela J. McLean,Guy A. Caldwell,Guy A. Caldwell,Ellen Sidransky,Gregory A. Grabowski,Gregory A. Grabowski,Dimitri Krainc +12 more
TL;DR: It is shown that functional loss of GD-linked glucocerebrosidase in primary cultures or human iPS neurons compromises lysosomal protein degradation, causes accumulation of α-synuclein (α-syn), and results in neurotoxicity through aggregation-dependent mechanisms, suggesting that GCase depletion contributes to the pathogenesis of sporadic synucleinopathies.
Journal ArticleDOI
Extrinsic fluorescent dyes as tools for protein characterization.
TL;DR: The intention of this review is to give an overview of available extrinsic dyes, explain their spectral properties, and show illustrative examples of their various applications in protein characterization.
References
More filters
Journal ArticleDOI
Determination of the absolute quantum yield of fluorescent solutions
Gregorio Weber,F. W. J. Teale +1 more
Journal ArticleDOI
Studies on the structure of hemoglobin I. Physicochemical properties of human globin
TL;DR: The most important physicochemical constants of human globin and its coupling capacity for hematin are reported.
Journal ArticleDOI
Fragmentation of bovine serum albumin by pepsin. i. the origin of the acid expansion of the albumin molecule.
Gregorio Weber,Lorna B. Young +1 more
Journal ArticleDOI
Amino-Acid Sequence of Sperm Whale Myoglobin
Journal ArticleDOI
Reversible conformational changes of myoglobin and apomyoglobin.
TL;DR: Data is reported which indicates that a small loss in helix content, which is recovered on reconstitution, accompanies removal of the heme prosthetic group from myoglobin, and that apomyoglobin, restored in this manner to its native conformation, is capable of combining with heme to form myoglobin.