Journal ArticleDOI
In vitro transcription of adenovirus 2 DNA by Escherichia coli RNA polymerase
Ulf Pettersson,Ulf Pettersson,Joseph Sambrook,Joseph Sambrook,Hajo Delius,Hajo Delius,Clark Tibbetts,Clark Tibbetts +7 more
Reads0
Chats0
TLDR
Competition hybridization experiments show that most of the viral sequences which are represented in stable RNA late during lytic adenovirus infection are transcribed by E. coli RNA polymerase in vitro.About:
This article is published in Virology.The article was published on 1974-05-01. It has received 13 citations till now. The article focuses on the topics: RNA-dependent RNA polymerase & Coding strand.read more
Citations
More filters
Book
Molecular Biology of Adenoviruses
TL;DR: In the short interval between 1953 and 1956 the adenoviruses were discovered, methods for laboratory diagnosis and serotyping were established, the epidemiology was clarified and a highly effective vaccine was developed and approved (for a review see Hilleman, 1966).
Journal ArticleDOI
Specific Fragmentation of DNA of Adenovirus Serotypes 3, 5, 7, and 12, and Adeno-Simian Virus 40 Hybrid Virus Ad2+ND1 by Restriction Endonuclease R· EcoRI
TL;DR: The products of complete digestion of duplex DNA of each of seven human adenoviruses with restriction endonuclease R were analyzed in this article, and the products ranged from two fragments for Adenovirus 7 DNA (Ad7) to six fragments for ad12 and Ad2 DNA.
Journal ArticleDOI
Characterization of adenovirus type 2 transcriptional complexes isolated from infected HeLa cell nuclei.
TL;DR: The analysis of the RNA synthesized from partially purified transcriptional complexes supports the contention that its transcription is almost entirely asymmetric, and that the asymmetry observed in vivo is not a consequence of the rapid degradation of h-strand transcripts.
Journal ArticleDOI
Epstein-Barr virus DNA in human lymphoid cell lines: in vitro conversion.
Maria Andersson,Tomas L. Lindahl +1 more
TL;DR: Two different nucleic acid hybridization techniques were used to search for Epstein-Barr virus (EBV) DNA in four human lymphoma-derived cell lines of B lymphocyte type, finding no EBV-associated nuclear antigen, EBNA, and four stably EBNA-positive sublines contained EBV DNA sequences.
References
More filters
Journal ArticleDOI
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane
David Gillespie,Sol Spiegelman +1 more
TL;DR: An improved method for the formation of DNA—RNA hybrids is described, quantitatively more certain than annealing in liquid, since the competing DNA renaturation reaction is suppressed.
Journal ArticleDOI
Factor stimulating transcription by RNA polymerase.
TL;DR: A protein component usually associated with RNA polymerase can be separated from the enzyme by chromatography on phosphocellulose, and the polymerase is unable to transcribe T4 DNA unless this factor is added back.
Journal ArticleDOI
A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
TL;DR: A method for the purification of Escherichia coli DNA-dependent RNA polymerase which is rapid, reproducible, high in yield, and able to handle preparations using from 1 g to 3 kg of cells is described.
Journal ArticleDOI
Studies of Simian Virus 40 DNA VII. A Cleavage Map of the SV40 Genome
TL;DR: A physical map of the Simian virus 40 genome has been constructed on the basis of specific cleavage of Simianirus 40 DNA by bacterial restriction endonucleases and the single site in SV40 DNA cleaved by the Escherichia coli RI restrictions endonuclease has been located.