Journal ArticleDOI
Independent mechanisms involved in suppression of the moloney leukemia virus genome during differentiation of murine teratocarcinoma cells
TLDR
Two independent mechanisms seem to regulate gene expression during the course of differentiation: the first mechanism operates in undifferentiated cells to block expression of M-MuLV and other exogeneously acquired viral genes, such as SV40 and polyoma virus, and does not depend on DNA methylation.About:
This article is published in Cell.The article was published on 1983-04-01. It has received 185 citations till now. The article focuses on the topics: DNA methylation & Cellular differentiation.read more
Citations
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Journal ArticleDOI
DNA methylation patterns and epigenetic memory
TL;DR: The heritability of methylation states and the secondary nature of the decision to invite or exclude methylation support the idea that DNA methylation is adapted for a specific cellular memory function in development.
Journal ArticleDOI
CpG-rich islands and the function of DNA methylation
TL;DR: It is likely that most vertebrate genes are associated with ‘HTF islands’—DNA sequences in which CpG is abundant and non-methylated; however, highly tissue-specific genes, though, usually lack islands.
Journal ArticleDOI
S-Adenosylmethionine and methylation.
Petek K. Chiang,Richard K. Gordon,Jacov Tal,G. C. Zeng,B. P. Doctor,K. Pardhasaradhi,Peter P. McCann +6 more
TL;DR: S‐Adenosylmethionine (AdoMet or SAM) plays a pivotal role as a methyl donor in a myriad of biological and biochemical events and although it has been claimed that AdoMet itself has therapeutic benefits, it remains to be established whether it can be taken up intact by cells.
Journal ArticleDOI
Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET
Matsui Toshiyuki,Danny Leung,Hiroki Miyashita,Irina A. Maksakova,Hitoshi Miyachi,Hiroshi Kimura,Makoto Tachibana,Matthew C. Lorincz,Yoichi Shinkai +8 more
TL;DR: It is proposed that a DNA-methylation-independent pathway involving KAP1 and ESET/ESET-mediated H3K9me3 is required for proviral silencing during the period early in embryogenesis when DNA methylation is dynamically reprogrammed.
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Functional Integration of Electrically Active Cardiac Derivatives From Genetically Engineered Human Embryonic Stem Cells With Quiescent Recipient Ventricular Cardiomyocytes Insights Into the Development of Cell-Based Pacemakers
Tian Xue,Hee Cheol Cho,Fadi G. Akar,Suk Ying Tsang,Steven P. Jones,Eduardo Marbán,Gordon F. Tomaselli,Ronald A. Li +7 more
TL;DR: It is demonstrated that electrically active, donor CMs derived from hESCs that had been stably genetically engineered by a recombinant lentivirus can functionally integrate with otherwise-quiescent, recipient, ventricular CMs to induce rhythmic electrical and contractile activities in vitro.
References
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
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Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
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Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
TL;DR: A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.
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Selective extraction of polyoma DNA from infected mouse cell cultures.
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Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange
TL;DR: A simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis, using the metachromatic stain acridine orange for visualization ofucleic acids in gels and which can be analyzed on the same slab gel.