Integrin-associated protein : a 50-kd plasma membrane antigen physically and functionally associated with integrins
Reads0
Chats0
TLDR
The data demonstrate an association between integrins and the 50-kD protein on several cell types and hypothesize that IAP may play a role in signal transduction for enhanced phagocytosis by Arg-Gly-Asp ligands.Abstract:
Phagocytosis by monocytes or neutrophils can be enhanced by interaction with several proteins or synthetic peptides containing the Arg-Gly-Asp sequence. Recently we showed that an mAb, B6H12, specifically inhibited this enhancement of neutrophil phagocytosis by inhibiting Arg-Gly-Asp binding to the leukocyte response integrin (Gresham, H. D., J. L. Goodwin, P. M. Allen, D. C. Anderson, and E. J. Brown. 1989. J. Cell Biol. 108:1935-1943). Now, we have purified the antigen recognized by B6H12 to homogeneity. Surprisingly, it is a 50-kD molecule that is expressed on the plasma membranes of all hematopoietic cells, including erythrocytes, which express no known integrins. On platelets and placenta, but not on erythrocytes, this protein is associated with an integrin that can be recognized by an anti-beta 3 antibody. In addition, both the anti-beta 3 and several mAbs recognizing the 50-kD protein inhibit Arg-Gly-Asp stimulation of phagocytosis. These data demonstrate an association between integrins and the 50-kD protein on several cell types. For this reason, we call it Integrin-associated Protein (IAP). We hypothesize that IAP may play a role in signal transduction for enhanced phagocytosis by Arg-Gly-Asp ligands.read more
Citations
More filters
Journal ArticleDOI
Integrins: versatility, modulation, and signaling in cell adhesion.
Journal ArticleDOI
Cellular functions regulated by src family kinases
Sheila M. Thomas,Joan S. Brugge +1 more
TL;DR: This chapter reviews the evidence implicating Src family kinases in specific receptor pathways and describes the mechanisms leading to their activation, the targets that interact with these kinases, and the biological events that they regulate.
Journal ArticleDOI
Signal transduction from the extracellular matrix.
Rudy L. Juliano,Stephen Haskill +1 more
TL;DR: There are many compelling examples of control of cell differentiation and gene expression through adhesive interactions with extracellular matrix, including activation of T-lymphocytes through the T-cell antigen receptor is markedly enhanced by integrin-mediated adhesion to fibronectin or laminin.
Journal ArticleDOI
Role of CD47 as a marker of self on red blood cells.
Per-Arne Oldenborg,Alex Zheleznyak,Yi-Fu Fang,Carl F. Lagenaur,Hattie D. Gresham,Frederik P. Lindberg +5 more
TL;DR: It is shown that CD47 (integrin-associated protein) functions as a marker of self on murine red blood cells and may represent a potential pathway for the control of hemolytic anemia.
Journal ArticleDOI
The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors
Stephen B. Willingham,Jens Peter Volkmer,Andrew J. Gentles,Debashis Sahoo,Piero Dalerba,Siddhartha Mitra,Jian Wang,Humberto Contreras-Trujillo,Robin Martin,Justin D. Cohen,Patricia Lovelace,Ferenc A. Scheeren,Mark P. Chao,Kipp Weiskopf,Chad Tang,Anne Kathrin Volkmer,Tejaswitha J. Naik,Theresa A. Storm,Adriane Mosley,Badreddin Edris,Seraina Schmid,Chris K. Sun,Mei-Sze Chua,Oihana Murillo,Pradeep S. Rajendran,Adriel C. Cha,Robert K. Chin,Dongkyoon Kim,Maddalena Adorno,Tal Raveh,Diane Tseng,Siddhartha Jaiswal,Per Øyvind Enger,Gary K. Steinberg,Gordon Li,Samuel So,Ravindra Majeti,Griffith R. Harsh,Matt De Van Rijn,Nelson N.H. Teng,John B. Sunwoo,Ash A. Alizadeh,Michael F. Clarke,Irving L. Weissman +43 more
TL;DR: All human solid tumor cells require CD47 expression to suppress phagocytic innate immune surveillance and elimination, showing that CD47 is a commonly expressed molecule on all cancers, its function to blockphagocytosis is known, and blockade of its function leads to tumor cell phagcytosis and elimination.
References
More filters
Journal ArticleDOI
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
TL;DR: A rapid and convenient method for peptide mapping of proteins has been developed that involves partial enzymatic proteolysis in the presence of sodium dodecyl sulfate and analysis of the cleavage products by polyacrylamide gel electrophoresis.
Journal ArticleDOI
The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins.
Book ChapterDOI
Polyacrylamide Gel Electrophoresis of Viral Proteins
TL;DR: This chapter describes polyacrylamide gel electrophoresis of viral proteins in gels, which are extensively used at the present time and are more homogeneous and stable than other commonly used materials.
Journal ArticleDOI
Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression.
TL;DR: Investigating the effects of ligation of the fibronectin receptor (FnR) on gene expression in rabbit synovial fibroblasts demonstrated that signals leading to changes in gene expression are transduced by the FnR, a member of the integrin family of extracellular matrix receptors.
Journal ArticleDOI
Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril.
Markwell Ma,Fox Cf +1 more
TL;DR: The effectiveness, simplicity of use, and versatility of chloroglycoluril recommend it as an iodinating reagent for both surface-specific and nonvectorial labeling of membrane systems.