Interactions between the vascular peptide endothelin-1 and sensory neuropeptides in gastric mucosal injury.
B.J.R. Whittle,J. Lopez-Belmonte +1 more
Reads0
Chats0
TLDR
Interactions between ET‐1 and sensory neuropeptides, which may reflect an important influence of these peptide mediators in the regulation of mucosal integrity, are suggested.Abstract:
1. The interactions between endogenous and exogenous sensory neuropeptides on gastric mucosal injury induced by endothelin-1 (ET-1) have been investigated in the anaesthetized rat. 2. Close intra-arterial infusion of ET-1 (4-20 pmol kg-1 min-1) dose-dependently induced vasocongestion and haemorrhagic necrosis in the gastric mucosa. 3. Capsaicin-pretreatment, two weeks earlier to deplete sensory neuropeptides from primary afferent neurones, augmented the mucosal damage induced by ET-1, as assessed by both macroscopic and histological examination. 4. The damage induced by threshold doses of ET-1 alone or in capsaicin-pretreated rats was further enhanced by administration of indomethacin (5 mg kg-1, i.v.), indicating a modulatory influence of endogenous prostanoids. 5. Morphine administration (3 mg kg-1, i.v.), which can prevent neuropeptide release, augmented the damage induced by threshold doses of ET-1, this effect being reversed by naloxone (1 mg kg-1, i.v.). 6. Concurrent local intra-arterial infusion of rat alpha-calcitonin gene-related peptide (10-50 pmol kg-1 min-1) dose-dependently reduced the mucosal injury induced by ET-1. 7. These findings suggest interactions between ET-1 and sensory neuropeptides, which may reflect an important influence of these peptide mediators in the regulation of mucosal integrity.read more
Citations
More filters
Journal ArticleDOI
Neural emergency system in the stomach
TL;DR: The pathophysiological potential of the neural emergency system is best portrayed by the gastric hyperemic response to acid backdiffusion, which is signaled by afferent nerve fibers and creates favorable conditions for rapid restitution and healing of the wounded mucosa.
Book ChapterDOI
Peptidergic sensory neurons in the control of vascular functions: mechanisms and significance in the cutaneous and splanchnic vascular beds.
Journal ArticleDOI
The actions of nitric oxide donors in the prevention or induction of injury to the rat gastric mucosa.
TL;DR: It is suggested that exogenous NO can protect the rat gastric mucosa from damage induced by the vasoconstrictor peptide ET‐1, which may reflect local microcirculatory interactions.
Journal ArticleDOI
Neuronal and endothelium-derived mediators in the modulation of the gastric microcirculation : integrity in the balance
TL;DR: It will become apparent that local neuronal and endotheliumderived factors that affect the microcirculation have a crucial influence on the processes that allow the gastric mucosa to regulate microvascular blood flow in the gastic mucosa.
Journal ArticleDOI
Endogenous nitric oxide and sensory neuropeptides interact in the modulation of the rat gastric microcirculation.
B.L. Tepperman,B.J.R. Whittle +1 more
TL;DR: It is shown that endogenous sensory neuropeptides and NO can interact in the regulation of the gastric microcirculation and the effects of l‐NAME on BP were not altered by capsaicin and indomethacin administration.
References
More filters
Journal ArticleDOI
A novel potent vasoconstrictor peptide produced by vascular endothelial cells.
Masashi Yanagisawa,Hiroki Kurihara,Hiroki Kurihara,Sadao Kimura,Yoko Tomobe,Mieko Kobayashi,Youji Mitsui,Yoshio Yazaki,Katsutoshi Goto,Tomoh Masaki +9 more
TL;DR: Cloning and sequencing of preproendothelin complementary DNA shows that mature endothelin is generated through an unusual proteolytic processing, and regional homologies to a group of neurotoxins suggest that endothelins is an endogenous modulator of voltage-dependent ion channels.
Journal ArticleDOI
The human endothelin family: three structurally and pharmacologically distinct isopeptides predicted by three separate genes.
Akihiro Inoue,Masashi Yanagisawa,Sadao Kimura,Yoshitoshi Kasuya,Takashi Miyauchi,Katsutoshi Goto,Tomoh Masaki +6 more
TL;DR: Three distinct human endothelin-related genes were cloned by screening a genomic DNA library under a low hybridization stringency with a synthetic oligonucleotide probe encoding a portion of the endotheli sequence, suggesting the possible existence of endothelins receptor subtypes.
Journal ArticleDOI
Calcitonin gene-related peptide is a potent vasodilator
TL;DR: It is suggested that local extravascular release of CGRP may be involved in the physiological control of blood flow and that circulating C GRP may contribute to hyperaemia in certain pathological conditions.
Journal ArticleDOI
Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor
Tai Kubo,Kazuhiko Fukuda,Atsushi Mikami,Akito Maeda,Hideo Takahashi,Masayoshi Mishina,Tatsuya Haga,Kazuko Haga,Arata Ichiyama,Kenji Kangawa,Masayasu Kojima,Hisayuki Matsuo,Tadaaki Hirose,Shosaku Numa +13 more
TL;DR: Cloning and sequence analysis of DNA complementary to porcine cerebral messenger RNA encoding the muscarinic acetylcholine receptor predict the complete amino-acid sequence of this protein.
Journal ArticleDOI
Regulation of gastric mucosal integrity by endogenous nitric oxide: interactions with prostanoids and sensory neuropeptides in the rat.
TL;DR: A role for endogenous NO formed from l‐arginine, acting in concert with prostacyclin and sensory neuropeptides, in the modulation of gastric mucosal integrity is suggested.
Related Papers (5)
Gastric mucosal protection against ulcerogenic factors in the rat mediated by capsaicin-sensitive afferent neurons
Peter Holzer,W. Sametz +1 more
Stimulation of afferent nerve endings by intragastric capsaicin protects against ethanol-induced damage of gastric mucosa.
Peter Holzer,I.Th. Lippe +1 more
Characterization of the peptidergic afferent innervation of the stomach in the rat, mouse and guinea-pig
T. Grhkn,Graham J. Dockray +1 more