Journal ArticleDOI
Interferon, double-stranded RNA and RNA degradation. Fractionation of the endonucleaseINT system into two macromolecular components; role of a small molecule in nuclease activation.
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TLDR
The separation and partial purification of two macromolecular components involved in the incubation of an extract from interferon-treated Ehrlich ascites tumor cells with double-straded RNA and ATP are reported.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1978-04-14. It has received 117 citations till now. The article focuses on the topics: Nuclease protection assay & Nuclease.read more
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Interferon action: RNA cleavage pattern of a (2'-5')oligoadenylate--dependent endonuclease.
TL;DR: The most frequent ribonuclease L cleavages occur after UA, UG, and UU (A, adenine; U, uracil; and G, guanine) and much less frequent cleavage after CA and AC (C, cytosine).
Journal ArticleDOI
The 2-5A system: modulation of viral and cellular processes through acceleration of RNA degradation.
Mark R. Player,Paul F. Torrence +1 more
TL;DR: Additional possible biological roles of the 2–5A system, such as in cell growth and differentiation, human immunodeficiency virus replication, heat shock, atherosclerotic plaque, pathogenesis of Type I diabetes, and apoptosis, are presented.
Journal ArticleDOI
New Insights into the Role of RNase L in Innate Immunity
TL;DR: A broader role in innate immunity is suggested by involvement of RNase L in cytokine induction and endosomal pathways that suppress bacterial infections and could eventually provide the basis for developing broad-spectrum antimicrobial drugs.
Journal ArticleDOI
Engagement of the cellular receptor for glycoprotein B of human cytomegalovirus activates the interferon-responsive pathway.
TL;DR: A principal means by which cytomegalovirus induces intracellular signaling and activation of the interferon-responsive pathway is via an interaction of gB with an as yet unidentified, likely novel cellular receptor that interfaces with the IFN signaling pathway.
Journal ArticleDOI
The (2′-5′)Oligoadenylate (pppA2′-5′A2′-5′A) Synthetase and Protein Kinase(s) from Interferon-Treated Cells
Ara G. Hovanessian,Ian M. Kerr +1 more
TL;DR: Two enzyme activities have been partially purified from interferon-treated mouse L cells: the (2′-5′)An synthetase and the protein kinases responsible for the phosphorylation of a polypeptide of molecular weight 67000 and the 35000-Mr subunit of the protein synthesis initiation factor eIF-2.
References
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Journal ArticleDOI
Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels
A C Peacock,C W Dingman +1 more
Journal ArticleDOI
Phosphorylation of initiation factor eIF-2 and the control of reticulocyte protein synthesis
TL;DR: Several lines of evidence are presented in support of the hypothesis that the phosphorylation of elF-2 by these kinases is basis of the control of initiation in lysates incubated under these conditions.
Journal ArticleDOI
Interferon-mediated protein kinase and low-molecular-weight inhibitor of protein synthesis
Walden K. Roberts,Walden K. Roberts,Ara G. Hovanessian,Ronald E. Brown,Michael J. Clemens,Michael J. Clemens,Ian M. Kerr +6 more
TL;DR: The presence of a dsRNA-dependent protein kinase activity in interferon cell sap is reported, which does not correspond either to the major polypeptide product of theinterferon-dsRNA specific phosphorylation, or, presumably, to phosphorylated IF-E2.
Journal ArticleDOI
Specific phosphorylation in vitro of a protein associated with ribosomes of interferon-treated mouse L cells.
TL;DR: It is shown that in the subcellular fraction which contains the interferoninduced inhibitor(s) of translation, there is strong inhibition of polypeptide chains in the form of protein phosphorylation.
Journal ArticleDOI
Antiviral activity of interferons.
TL;DR: Interferon Treatment Inhibits Terminal Events in the Replication Cycle of Murine Leukemia Viruses and Transcription of the Viral Genome.
Related Papers (5)
Interferon action may be mediated by activation of a nuclease by pppA2'p5'A2'p5'A.
Inhibition of Cell-Free Protein Synthesis by pppA2'p5'A2'p5' A: a Novel Oligonucleotide Synthesized by Interferon-Treated L Cell Extracts
pppA2'p5'A2'p5'A: an inhibitor of protein synthesis synthesized with an enzyme fraction from interferon-treated cells
Ian M. Kerr,Ronald E. Brown +1 more