Involvement of sigma(S) in starvation-induced transposition of Pseudomonas putida transposon Tn4652.
TLDR
Evidence that transposition of Tn4652 is elevated in stationary-phase Pseudomonas putida and suppressed in an isogenic ςS-defective strain is presented, and it is demonstrated that transcription from the Tn 4652 transposase promoter is controlled by the stationary- phase-specific sigma factor ρS.Abstract:
Transpositional activity of mobile elements can be induced by different environmental stresses. Here, we present evidence that transposition of Tn4652 is elevated in stationary-phase Pseudomonas putida and suppressed in an isogenic sigma(S)-defective strain. We demonstrate that transcription from the Tn4652 transposase promoter is controlled by the stationary-phase-specific sigma factor sigma(S). To our knowledge, this is the first example of direct stationary-phase-specific regulation of a mobile element transposase. Data presented in this report support the idea that activation of transposition under stressful conditions could be an inducible process.read more
Citations
More filters
Journal ArticleDOI
Stress-Induced Mutagenesis in Bacteria
Ivana Bjedov,Ivana Bjedov,Olivier Tenaillon,Olivier Tenaillon,Bénédicte Gérard,Bénédicte Gérard,Valeria Souza,Valeria Souza,Erick Denamur,Erick Denamur,Miroslav Radman,Miroslav Radman,François Taddei,François Taddei,Ivan Matic,Ivan Matic +15 more
TL;DR: It is suggested that irrespective of the causes of their emergence, stress-induced mutations participate in adaptive evolution and could be a by-product of genetic strategies for improving survival under stress.
Journal ArticleDOI
Fate and transport of antibiotic residues and antibiotic resistance genes following land application of manure waste.
Joanne C. Chee-Sanford,Roderick I. Mackie,Satoshi Koike,Ivan G. Krapac,Yu-Feng Lin,Anthony C. Yannarell,Scott Maxwell,Rustam Aminov +7 more
TL;DR: Findings are discussed that address aspects of the fate, transport, and persistence of antibiotics and antibiotic resistance genes in natural environments, with emphasis on mechanisms pertaining to soil environments following land application of animal waste effluent.
Journal ArticleDOI
Mutation as a Stress Response and the Regulation of Evolvability
TL;DR: The emerging commonalities in stress-induced-mutation mechanisms provide hope for new therapeutic interventions for all of these processes, including microbial pathogenesis and antibiotic-resistance, and tumor progression and chemotherapy resistance, all of which occur under stress, driven by mutations.
Book
Horizontal Gene Exchange in Environmental Microbiota
TL;DR: A brief account of the occurrence and diversity of MGEs in natural ecosystems and of the environmental factors that may affect MGE-mediated HGT is given.
Journal ArticleDOI
Evolution and ecology of antibiotic resistance genes
Rustam Aminov,Roderick I. Mackie +1 more
TL;DR: Phylogenetic insights into the evolution and diversity of several antibiotic resistance genes suggest that at least some of these genes have a long evolutionary history of diversification that began well before the 'antibiotic era'.
References
More filters
Journal ArticleDOI
Transposable elements as sources of variation in animals and plants.
Margaret G. Kidwell,Damon Lisch +1 more
TL;DR: Here, TE-induced variation is viewed from three evolutionary perspectives that are not mutually exclusive and possible coadaptations between elements and their hosts that appear to have evolved to reduce the deleterious effects of new insertions on host fitness are described.
Journal ArticleDOI
The Role of the Sigma Factor sigmas (KatF) in Bacterial Global Regulation
TL;DR: Proteins in the regulon enhance long-term survival in nutrient-deficient medium and have a diverse group of functions including protection against DNA damage, the determination of morphological changes, the mediation of virulence, osmoprotection, and thermotolerance.
Journal ArticleDOI
Stress and transposable elements: co-evolution or useful parasites?
TL;DR: In this article, the authors discuss hypotheses based upon the existence of promoters or fixation sites of transcription activators in the untranslated regions of transposable elements, similar to those found in regulatory regions of host defence genes.
Journal ArticleDOI
Plant retrotransposons: Turned on by stress
TL;DR: All known active plant retrotransposons are largely quiescent during development but activated by stresses, including wounding, pathogen attack and cell culture, and could be the stress-induced generators of genomic diversity proposed by McClintock.
Journal ArticleDOI
Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors
TL;DR: A broad-host-range vector, pKT240, containing the structural gene (aph) for aminoglycoside phosphotransferase (APH), without promoter, has been constructed to show that the hybrid trp-lac (tac) promoter and the promoter of the lacIQ gene of Escherichia coli are active in Pseudomonas putida.